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Herculase 2 phusion polymerase

Manufactured by Agilent Technologies
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Sourced in Germany

Herculase II Phusion Polymerase is a high-fidelity DNA polymerase enzyme used for PCR amplification. It exhibits a proofreading activity, resulting in a low error rate during DNA synthesis.

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Lab products found in correlation

Oligonucleotide primers, by Thermo Fisher Scientific (1 mentions) Genejet pcr purification kit, by Thermo Fisher Scientific (1 mentions) Phusion high fidelity dna polymerase, by Thermo Fisher Scientific (1 mentions) Genejet plasmid miniprep kit, by Thermo Fisher Scientific (1 mentions) Second strand synthesis module, by New England Biolabs (1 mentions) Amicon ultra 15 centrifugal filter, by Merck Group (1 mentions) E gel ex agarose gel, by Thermo Fisher Scientific (1 mentions) Stbl3 cells, by Thermo Fisher Scientific (1 mentions) Steadylite plus, by PerkinElmer (1 mentions) Peg 8000, by Merck Group (1 mentions) Nextera xt, by Illumina (1 mentions) Dynabeads myone streptavidin c1, by Thermo Fisher Scientific (1 mentions) Rlt lysis buffer, by Qiagen (1 mentions) Turbo dnase, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Nextera, by Illumina (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions)

2 protocols using herculase 2 phusion polymerase

1

Bacterial Genomic DNA and Plasmid Isolation

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Genomic DNA was isolated from 2 mL of bacterial suspension as published previously (Cheng and Jiang, 2006 (link)). Plasmid DNA was purified using the GeneJET Plasmid MiniPrep Kit (Thermo Scientific, Austria). PCR fragments were amplified either by Phusion High-Fidelity DNA Polymerase (Thermo Scientific) or by Herculase II Phusion Polymerase (Agilent Technologies, Germany) according to the protocols provided by manufacturers. PCR fragments were purified with the GeneJET PCR Purification Kit (Thermo Scientific). Oligonucleotide primers (Life Technologies) used in the course of this study are listed in Table 1.
Tomek M.B., Neumann L., Nimeth I., Koerdt A., Andesner P., Messner P., Mach L., Potempa J.S, & Schäffer C. (2014). The S-layer proteins of Tannerella forsythia are secreted via a type IX secretion system that is decoupled from protein O-glycosylation. Molecular oral microbiology, 29(6), 307-320.
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2

Plasmid Purification and Transfection Workflow

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DNA plasmids were propagated in Stbl3 cells (Life Technologies). Plasmids were purified by endotoxin-free midiprep kit (Macherey-Nagel). DMEM, penicillin-streptomycin, Glutamax, OPTIMEM, Lipofectamine 2000, E-Gel EX Agarose Gels, and Dynabeads MyOne Streptavidin C1 were purchased from Life Technologies. Additional reagents: Herculase II Phusion Polymerase (Agilent), Maxima reverse transcriptase and RNaseA (Thermo-Fisher), Betaine and Fetal bovine serum (FBS) (Sigma), TURBO DNase (Ambion), Second Strand Synthesis Module (NEB), RLT lysis buffer (Qiagen), Agencourt AMPure XP (Beckman Coulter), and Zymoclean Gel DNA Recovery Kit (Zymo Research), In-Fusion (Clontech), Amicon Ultra-15 Centrifugal Filter (EMD-Millipore), Steadylite Plus (PerkinElmer), PEG-8000 (Sigma), Nextera and NexteraXT (Illumina), 50-cycle MiSeq and 75-cycle NextSeq (Illumina).
O'Connell D.J., Kolde R., Sooknah M., Graham D.B., Sundberg T.B., Latorre I., Mikkelsen T.S, & Xavier R.J. (2016). Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing. Cell systems, 2(5), 323-334.
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