Rabbit anti gfp tag polyclonal

Immunostaining of flat-prepped stage 16 Drosophila embryos was performed using the following primary antibodies: mouse anti-Fas 2 (1:100, clone 1D4, DHSB), mouse anti-axons CNS (1:100, BP 102, DHSB), mouse anti-Futsch (1:1000, 22c10, DHSB), rabbit anti-phospho-JNK (Thr¹⁸³ / Tyr¹⁸⁵) (1:100, Cell Signaling #9251), rabbit anti-Dcp-1 (Asp216) (1:100, Cell Signaling #9578), rabbit anti-GFP tag polyclonal (1:600, Thermo Fisher Scientific), rat anti-Elav (1:1000, clone 7E8A10, DHSB), mouse anti-Repo (1:100, clone 8D12 DHSB), mouse anti-Even-skipped (1:100, clone 3C10, DHSB), mouse anti-Engrailed (1:100, clone 4D9, DHSB) and TRITC-conjugated goat anti-HRP (1:200, Jackson ImmunoResearch #123-025-021).
The secondary antibodies used for detection were: Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 conjugate (A-11008), Goat anti-Rabbit IgG (H + L) Alexa Fluor 555 conjugate (A-21428), Goat anti-Mouse IgG (H + L) Alexa Fluor 488 conjugate (A-11001), Goat anti-Mouse IgG (H + L) Alexa Fluor 555 conjugate (A-21422) and Goat anti-Rat IgG (H + L) Alexa Fluor 555 conjugate (A-21434). All secondary antibodies were used in a dilution of 1:600 and were from Invitrogen.

Immunostaining of whole mount or flat-dissected stage 16–17 Drosophila embryos was performed using the following primary antibodies: mouse anti-Fas2 (1:100, clone 1D4, DHSB), rabbit anti-GFP tag polyclonal (1:600, Thermo Fisher Scientific), mouse anti-Repo (1:100, clone 8D12 DHSB) and rat anti-Elav (1:1000, clone 7E8A10 DHSB).
The secondary antibodies used for detection were: Goat anti-Rabbit IgG (H + L) Alexa Fluor 488 conjugate (A-11008), Goat anti-Mouse IgG (H + L) Alexa Fluor 555 conjugate (A-21422) and Donkey anti-Rat IgG (H = L) Alexa Fluor 555 conjugate (A-48270). All secondary antibodies were used in a dilution of 1:600 and were from Invitrogen.