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CHSP70 is a heat shock protein that functions as a molecular chaperone, assisting in the folding, assembly, and translocation of other proteins within the cell. It plays a crucial role in maintaining cellular homeostasis and protecting cells from various forms of stress.

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Lab products found in correlation

Psd95, by Cell Signaling Technology (2 mentions) Hsp27, by Cell Signaling Technology (2 mentions) Rabbit anti hsp90, by Cell Signaling Technology (2 mentions) Synapsin 1, by Cell Signaling Technology (2 mentions) Mouse anti ihsp70, by Enzo Life Sciences (2 mentions) Anti mouse igg and anti rabbit igg horseradish peroxidase conjugated abs, by Merck Group (2 mentions) Hsp40, by Cell Signaling Technology (2 mentions) Rabbit anti bdnf, by Santa Cruz Biotechnology (2 mentions) Mouse anti β actin, by Merck Group (2 mentions)

2 protocols using chsp70

1

Western Blot Immunodetection of Molecular Chaperones

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots were performed as previously described16 (link). Antibodies (Abs) were used as follows: rabbit anti-HSP90, cHSP70, HSP40, HSP27, HSF1, PSD95, Synapsin I (1:1000; Cell Signaling); mouse anti-iHSP70 (1:1000; Enzo Life Sciences), rabbit anti-BDNF (1:500; Santa Cruz Biotechnology); mouse anti-β-actin (1:10000; Sigma-Aldrich), rabbit anti-Synaptophysin (1:2000; Chemicon/Millipore), and anti-mouse IgG and anti-rabbit IgG horseradish peroxidase-conjugated Abs (1:5000; Sigma-Aldrich).
Wang B., Liu Y., Huang L., Chen J., Li J.J., Wang R., Kim E., Justicia C., Sakata K., Chen H., Planas A., Ostrom R.S., Li W., Yang G., McDonald M.P., Chen R., Heck D, & Liao F.F. (2016). A CNS-permeable Hsp90 inhibitor rescues synaptic dysfunction and memory loss in APP-overexpressing Alzheimer’s mouse model via an HSF1-mediated mechanism. Molecular psychiatry, 22(7), 990-1001.
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2

Western Blot Immunodetection of Molecular Chaperones

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots were performed as previously described16 (link). Antibodies (Abs) were used as follows: rabbit anti-HSP90, cHSP70, HSP40, HSP27, HSF1, PSD95, Synapsin I (1:1000; Cell Signaling); mouse anti-iHSP70 (1:1000; Enzo Life Sciences), rabbit anti-BDNF (1:500; Santa Cruz Biotechnology); mouse anti-β-actin (1:10000; Sigma-Aldrich), rabbit anti-Synaptophysin (1:2000; Chemicon/Millipore), and anti-mouse IgG and anti-rabbit IgG horseradish peroxidase-conjugated Abs (1:5000; Sigma-Aldrich).
Wang B., Liu Y., Huang L., Chen J., Li J.J., Wang R., Kim E., Justicia C., Sakata K., Chen H., Planas A., Ostrom R.S., Li W., Yang G., McDonald M.P., Chen R., Heck D, & Liao F.F. (2016). A CNS-permeable Hsp90 inhibitor rescues synaptic dysfunction and memory loss in APP-overexpressing Alzheimer’s mouse model via an HSF1-mediated mechanism. Molecular psychiatry, 22(7), 990-1001.
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