Concentration of protein samples were determined using Pierce BCA protein assay kit (Thermo Fisher Scienfitic, USA). Samples were separated by 10% SDS-PAGE and transferred to PVDF membrane, and proteins were detected by first and second antibodies (Cell Signal Technology, Germany) with Western blot.
First and second antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
First and second antibodies are laboratory reagents used in various immunoassays and immunological techniques. The first antibody (primary antibody) binds to a specific target protein or antigen, while the second antibody (secondary antibody) binds to the first antibody, enabling detection and amplification of the signal. These antibodies are essential tools for researchers in fields such as cell biology, biochemistry, and molecular biology.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Genistein, by Merck Group (1 mentions)
Matrigel, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Dulbecco s modified eagle s medium, by Lonza (1 mentions)
Crystal violet stain solution, by Beyotime (1 mentions)
Penicillin and streptomycin 100, by Solarbio (1 mentions)
Ifluor 488 conjugated phalloidin, by Abcam (1 mentions)
Sds page electrophoresis solution, by Beyotime (1 mentions)
Magnolol, by Selleck Chemicals (1 mentions)
Matrigel, by Corning (1 mentions)
Dulbecco s modified eagle s medium dmem, by Corning (1 mentions)
3 protocols using first and second antibodies
1
Quantitative Protein Analysis by SDS-PAGE
2
Genistein-Induced Apoptosis in Cell Lines
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Dulbecco's modified Eagle's Medium (DMEM), 100× penicillin and streptomycin, and fetal bovine serum (FBS) were acquired from Lonza (Verviers, Belgium). The Annexin-V–PI Apoptosis Kit and TUNEL Apo-Green Detection Kit were purchased from Biotool (Shanghai, China). DMSO and genistein were both purchased from Sigma-Aldrich (Shanghai, China), and a stock solution (200 mM) of genistein was prepared in DMSO. Matrigel™ was acquired from Invitrogen (Karlsruhe, Germany), and WST-8 and the Phosphatase and Protease Inhibitor Cocktail Set I were acquired from Biotool. First and second antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Detailed information regarding the antibodies we used are listed in Table 1 . The enhanced chemiluminescence (ECL) detection system was obtained from Biotool.
3
Transwell Invasion Assay with Magnolol
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Dulbecco’s modified Eagle’s Medium (DMEM), Transwell nesting (with polycarbonate film 605 mm 8.0 μm), and Matrigel™ were purchased from Corning (NY, USA). Fetal bovine serum (FBS) was purchased from Tianhang (Huzhou, China). Dimethyl sulfoxide (DMSO) and 0.25% trypsin-EDTA (1X) were purchased from Sigma-Aldrich (Shanghai, China). The penicillin and streptomycin (100×) was purchased from Solarbio (Beijing, China). Magnolol and 5-FU were purchased from Selleck (Shanghai, China). Cell counting kit-8 (CCK-8), Phosphate buffer solution (PBS), Western transfer solution, SDS-PAGE electrophoresis solution and Crystal violet stain solution were purchased from Beyotime (Shanghai, China). iFluor 488-conjugated phalloidin was purchased from Abcam (Shanghai, China). First and second antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
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