N lauroylsarcosine sodium

N-lauroylsarcosine sodium, 3-aminopropyltriethoxysilane (APTES), tetraethyl orthosilicate (TEOS), ammonium acetate, 5-fluorouracil, and dexamethasone were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Carbopol 934 was purchased from Acros Organics (Morris Plains, NJ, USA). All other chemical reagents used were of analytical grade and used without further purification. HT-144 (ATCC^® HTB-63™), and McCoy’s 5a Medium Modified (ATCC^® 30-2007™) was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Medium culture Lysogeny Broth (LB) and LB-agar were purchased from Difco. Detergent n-dodecyl-ß-D-maltoside (DDM) was purchased from Anatrace, N-Lauroylsarcosine sodium (SLS) and Tween 20 from Sigma. The strains used in this study are listed in the Supplementary Table S1. Briefly, TOP10 and DH5α strains were used for cloning procedures, TOP10 and BL21 strains for protein production and DHM1 strains for bacterial two-hybrid assays. E. coli strains were routinely grown in LB medium at 37 °C and on LB-agar for solid culture. Plasmids were maintained by the addition of antibiotics such as ampicillin (100 µg/ml), kanamycin (50 µg/ml), chloramphenicol (50 µg/ml) and streptomycin (100 µg/ml).

N-Lauroylsarcosine sodium, 3-aminopropyltriethoxysilane (APTES), tetraethyl orthosilicate (TEOS), ammonium acetate, 5-fluorouracil, and dexamethasone were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Carbomer (Carbopol 934) was purchased from Acros Organics (Morris Plains, NJ, USA). O-Carboxymethyl chitosan (CMC) M. WT (100–300 KD) was purchased from Santacruz Biotechnology (Dallas, TX, USA). Mucin powder was supplied by Xian Kono Chem Co., Ltd. (Xi’an, China). All other chemical reagents used were of analytical grade and used without further purification.