Human cxcl12 sdf 1 immunoassay

For the quantitative determination of human cytokine concentrations, enzyme-linked immunosorbent assay was performed using The Quantikine Human HGF Immunoassay (R&D Systems, Inc. Minneapolis, MN, USA), Human CCL2/MCP-1 Immunoassay (R&D Systems, Inc.), Human CCL7/MCP-3 Immunoassay (R&D Systems, Inc.), Human Total MMP-3 Immunoassay (R&D Systems, Inc.), and Human CXCL12/SDF-1 Immunoassay (R&D Systems, Inc.) according to the manufacturer’s instructions. The ELISA data were obtained from four independent SHED, DPSCs, and BMMSCs, respectively, each of which was performed four times.

Blood samples were obtained from the patients from the antecubital vein between 7 and 8 am following an overnight fast. They were centrifuged at 4°C and subsequently frozen and stored at −70°C.
SDF-1 was quantified using a commercially available EIA kit (Human CXCL12/SDF-1 Immunoassay; R&D Systems Europe Ltd., Abingdon, UK; catalog no. DSA00) according to the manufacturer’s instructions. This kit is specific for the protein encoded by SDF-1 transcript variant 1, SDF-1α. Absorbance was read at 450 nm (BioTek Synergy 2; BioTek Instruments, Inc., Winooski, VT, USA). All steps were performed at room temperature.
CA 125 was quantified by the automated microparticle enzyme immunosorbent assay method using the AXSYM system of Abbott Laboratories (Chicago, IL, USA).