Anti cd38 apc

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-CD38 APC is a fluorescent-labeled antibody that binds to the CD38 cell surface antigen. CD38 is a transmembrane glycoprotein expressed on various cell types, including plasma cells and activated T and B cells. This antibody can be used for the detection and analysis of CD38-positive cells in flow cytometry applications.

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Lab products found in correlation

Anti cd45ra apc h7, by BD (2 mentions) Anti cd34 pe, by Beckman Coulter (2 mentions) Anti cd45 fitc, by Beckman Coulter (2 mentions) Anti lin fitc, by Thermo Fisher Scientific (2 mentions) Anti cd90 pe, by Thermo Fisher Scientific (2 mentions) Anti cd34 pe cy7, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Facscanto 2, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by BD (2 mentions) Stem count fluorospheres, by Beckman Coulter (1 mentions) Anti cd34 pe, by BD (1 mentions) Anti cd45 apc, by BD (1 mentions) Cd16 pe, by BioLegend (1 mentions) Anti cd123 pe, by BD (1 mentions) Anti cd34 fitc, by BD (1 mentions) Anti b220 pe, by Thermo Fisher Scientific (1 mentions) Anti gl7 fitc, by BioLegend (1 mentions) Dneasy blood and tissue kit, by Qiagen (1 mentions) Anti cd19 pe, by Thermo Fisher Scientific (1 mentions) Anti cd27 pe cy5, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD38 (7 citations) CD38-APC (6 citations) APC-conjugated anti-CD38 (3 citations) Anti-human CD38 APC (2 citations)

6 protocols using anti cd38 apc

Quantification of Human Hematopoietic Stem Cells

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Absolute numbers of viable human CD34⁺ cells were determined by a single platform flow cytometric assay using anti‐FITC‐CD45, anti‐CD34‐PE, and Stem‐Count Fluorospheres from the Stem‐Kit Reagents kit (Beckman Coulter) and DAPI (Sigma). The frequencies of human phenotypic HSCs were determined using anti‐Lin‐FITC, anti‐CD38‐APC, anti‐CD90‐PE (Thermo Fisher Scientific), anti‐CD34‐PE‐Cy7, anti‐CD45RA‐APC‐H7 (BD Biosciences), and DAPI. All samples were analyzed using BD FACSCanto II (BD Biosciences), and the data were analyzed using FlowJo software (Tree Star, Inc, Ashland, OR, USA).

Morhayim J., Ghebes C.A., Erkeland S.J., ter Borg M.N., Hoogenboezem R.M., Bindels E.M., van Alphen F.P., Kassem M., van Wijnen A.J., Cornelissen J.J., van Leeuwen J.P., van der Eerden B.C., Voermans C., van de Peppel J, & Braakman E. (2020). Identification of osteolineage cell‐derived extracellular vesicle cargo implicated in hematopoietic support. The FASEB Journal, 34(4), 5435-5452.

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Quantifying Human Hematopoietic Stem Cells

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Absolute numbers of viable human CD34⁺ cells were determined by a single platform flow cytometric assay using anti-FITC-CD45, anti-CD34-PE, and Stem-Count Fluorospheres - from the Stem-Kit Reagents kit (Beckman Coulter) and DAPI (Sigma). The frequencies of human phenotypic HSCs were determined using anti-Lin-FITC, anti-CD38-APC, anti-CD90-PE (Thermo Fisher Scientific), anti-CD34-PE-Cy7, anti-CD45RA-APC-H7 (BD Biosciences) and DAPI. All samples were analyzed using BD FACSCantoII (BD Biosciences), and the data was analyzed using FlowJo software (Tree Star, Inc., Ashland, OR, USA).

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Immunophenotyping of Bone Marrow Cells

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Immunophenotyping of bone marrow (BM) was performed using an FC500 or Facscalibur flow cytometer. FACS buffer was made with PBS+2mM EGTA+2% FBS. Primary AML and leukemic stem cell fractions were detected using the following antibodies (company; product #; clone): anti-CD34 PE (BD bio-sciences; 348057; 8G12), anti-CD34 FITC (BD Pharmingen; 555821; 581), anti-CD38 APC (ebiosciences; 17-0389-42; HIT2) and anti-CD123 PE (BD Pharmingen; 558714; 7G3), anti-CD45 APC (BD Pharmingen; 557513; TU116) and anti-class I HLA A, B, C (Biolegend; 311404; W6/32). NK-92 cells lines were assessed for CD16 expression using CD16 PE (Biolegend; 302008; 3G8). Leukemia cell lines were evaluated using anti-CD123 PErCy5.5 (BD Biosciences; 560904; 7G3). Cell sorting was performed using a FacsAria cell sorter as described in the Online Supplementary Methods.

Williams B.A., Wang X.H., Leyton J.V., Maghera S., Deif B., Reilly R.M., Minden M.D, & Keating A. (2018). CD16+NK-92 and anti-CD123 monoclonal antibody prolongs survival in primary human acute myeloid leukemia xenografted mice. Haematologica, 103(10), 1720-1729.

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Purification and FACS of mouse GC and non-GC B cells

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Spleen and PPs were dissected out from 8–12 weeks old mice, prepared into single cell suspensions and purified by EasySep® Negative Selection B cell Enrichment Kit (Stem Cell Techonologies) according to the manufacturer’s protocol. Purified B cells were stained with anti-B220-PE (1:2000) (eBiosciences), anti-CD38-APC (1:200) (eBiosciences) and anti-GL7-FITC (1:200) (Biolegend). GC (B220⁺GL7⁺CD38⁻) and nonGC (B220⁺GL7⁻CD38⁺) B cells were sorted from the same sample by fluorescence-activated cell sorting (FACS) in the Department of hematology/oncology flow cytometry research facility at Boston Children’s Hospital. Genomic DNA from sorted cells was prepared using a DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s protocol. 4 to 18 independent mice were analyzed for each category as indicated in the respective figures.

Chen H., Zhang Y., Ye A.Y., Du Z., Xu M., Lee C.S., Hwang J.K., Kyritsis N., Ba Z., Neuberg D., Littman D.R, & Alt F.W. (2020). BCR Selection and Affinity Maturation in Peyer’s Patches Germinal Centers. Nature, 582(7812), 421-425.

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Phenotypic Characterization of B Cell Subsets

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Peripheral venous whole blood was collected from subjects, and 0.83% ammonium
chloride was used to separate red blood cells. Whole blood was then stained with
the following fluorescent antibodies: anti-CD24-FITC, anti-CD19-PE,
anti-CD27-PEcy5, and anti-CD38-APC (eBioscience, San Diego, CA, USA).
Fluorescently-stained cells were then detected by BD FACSVerse flow cytometry
(BD Biosciences, San Jose, CA, USA). Peripheral venous blood B cells
(CD19⁺ lymphocytes) were divided into Breg cells
(CD19⁺CD24^hiCD38^hi), memory B cells
(CD19⁺CD27⁺CD24^hi), and plasma cells
(CD19⁺CD27^hiCD38^hi).

Guo S., Chen Q., Liang X., Mu M., He J., Fang Q., Song C, & Sang D. (2018). Reduced peripheral blood regulatory B cell levels are not associated with the Expanded Disability Status Scale score in multiple sclerosis. The Journal of International Medical Research, 46(9), 3970-3978.

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Profiling B Cell Subsets and Receptor Expression

Cited 1 time

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We processed PBMCs of the entire study population in four different batches. Frozen PBMCs were thawed, washed twice with PBS (Fisher Scientific) and stained with live/dead marker (Zombie Yellow™ Fixable Viability dye, eBioscience) and fluorochrome-conjugated antibodies against surface markers: anti-CD19 BV510, anti-CD24 BV421, anti-IgD APC-Cy7 (all BioLegend); anti-CD27 AF-700, anti-CD38 APC, anti-CD40 PE-Cy7, anti-BAFF-R FITC (all eBioscience); anti-CD86 PE-CF594, and anti-TACI BV650 (all BD Biosciences). A total of five B cell subpopulations could be measured using the following gating strategy as previously reported (18 (link)): total (CD19⁺), transitional (CD19⁺CD24^hiCD38^hi), naïve (CD19⁺CD27^-), unswitched memory (CD19⁺CD27⁺IgD⁺) and switched memory B cells (CD19⁺CD27⁺IgD^-). We measured expression of CD40, BAFF-R and TACI as percentage of positive cells for all five B cell subsets and as mean of fluorescence intensity (MFI) across positive cells for each cell type. The absolute cell counts of plasmablasts (CD19⁺CD24^-CD38^hi) were too low (< 100 cells) to reliably determine expression levels. Data were collected on BD Symphony flow cytometer (BD Biosciences). For data analysis, we used FlowJo (LLC, V10). Supplementary Figure 1 shows representative FACS plots.

Smets I., Prezzemolo T., Imbrechts M., Mallants K., Mitera T., Humblet-Baron S., Dubois B., Matthys P., Liston A, & Goris A. (2021). Treatment-Induced BAFF Expression and B Cell Biology in Multiple Sclerosis. Frontiers in Immunology, 12, 676619.

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