Human anti-RAB5, RAB7, and RAB11 antibodies (Rab Family Antibody Sampler Kit #9385) were acquired from Cell Signaling Technology (Danvers, MA, USA); human anti-GAPDH (sc-47724), anti-Calnexin (sc-11397) and anti-PARP (sc-7150) antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA); human anti-Tsg101 (#MA1-23296) antibody was from Invitrogen (Carlsbad, CA, USA); anti-Alix (ab186429) and anti-CD9 (ab92726) antibodies were acquired from Abcam (Cambridge, UK).
Anti parp sc 7150
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-PARP (sc-7150) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect PARP (Poly(ADP-ribose) Polymerase) protein expression in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Anti alix ab186429, by Abcam (1 mentions)
Anti cd9 ab92726, by Abcam (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Cytiva (1 mentions)
Anti akt 9272, by Cell Signaling Technology (1 mentions)
Anti lc3 4108, by Cell Signaling Technology (1 mentions)
Anti vimentin 5741, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by BD (1 mentions)
Anti γ tubulin, by Merck Group (1 mentions)
Anti lc3a b, by Cell Signaling Technology (1 mentions)
Anti gm130 12480, by Cell Signaling Technology (1 mentions)
Anti phospho p44 42 mapk t202 y204, by Cell Signaling Technology (1 mentions)
Anti caspase3 9662, by Cell Signaling Technology (1 mentions)
Anti actin, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti ucp1, by Merck Group (1 mentions)
4 protocols using anti parp sc 7150
1
Antibody Panel for Rab GTPase and Cell Markers
2
Extensive Antibody Characterization for Research
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The following antibodies were purchased: anti-phospho-AKT (S473) D9E #4060, anti-AKT #9272, anti-Caspase-3 #9662, anti-LC3 A/B (CST#4108), anti-vimentin #5741, anti-GM130 #12480, anti-phospho p44/42 MAPK (T202/Y204) #4370s, anti-LC3 #4108 (used for western blotting) from Cell Signaling Technology (Leiden, The Netherlands); anti-E-cadherin #610182 from BD Biosciences (San Jose, CA, USA); anti-calnexin #SPC-108 from Stress Marq Biosciences Inc. (Victoria, Canada); anti-CD107a/Lamp1 (clone H4A3) #SAB4700416, anti-αTubulin (clone B512) #T5168, anti-γtubulin #T6657 from Sigma-Aldrich (St. Louis, MI, USA); anti-GOLGIN-97 #A-21270 Thermo Fisher Scientific—Invitrogen, (Carlsbad, CA, USA); anti-LC3 (clone 5F10) #0231, used for immunofluorescence, from Nanotools (Teningen, Germany); anti-PFKM #a5477 from Abclonal Technology (Woburn, MA, USA); anti-PARP #sc-7150 from Santa Cruz Biotechnology, Inc (Santa Crus, CA, USA). Alexa-Fluor (488 and 546) secondary antibodies A11029, A11030, A11034, and A11035, were from Thermo Fisher Scientific—Invitrogen, (Carlsbad, CA, USA) and horseradish peroxidase (HRP)-conjugated secondary antibodies used for Western blot analyses were from Amersham Pharmacia (Buckinghamshire, UK).
3
Subcellular Fractionation and Western Blotting
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Subcellular fractionation and western blotting analyses were carried out as previously described (17) . Lysates were subjected to differential centrifugation to isolate the nuclear and mitochondrial fraction. Proteins were extracted from nuclei and mitochondria by incubation in boiling sample buffer followed by sonication. In HaCaT cell lysates, nuclear fractionation was achieved by incubation of nuclei in buffer C (420 mM NaCl, 1 mM EDTA, 20 mM HEPES pH 7.9, 25% glycerol, 1 mM protease inhibitors, 1 mM PMSF) for 20 min at 4 °C with agitation. After centrifugation for 15 min at 16,000 × g at 4 °C, supernatants (corresponding to nucleosol) were separated from nuclear pellets, which were resuspended in boiling sample buffer followed by sonication. Thirty µg of each fraction were separated using 10% SDS-PAGE and analysed by western blotting. Mouse anti-VDR (sc-13133) and rabbit antibody anti-PARP (sc-7150) were from Santa Cruz, CA, USA.
4
Subcellular Fractionation and Western Blotting
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Subcellular fractionation and Western blotting analysis was carried out as previously described [16] . Lysates were subjected to differential centrifugation to isolate the nuclear and mitochondrial fraction. Proteins were extracted by incubation in boiling sample buffer followed by sonication.
Fifty µg of total lysates or thirty µg of nuclear or mitochondrial fractions were separated by 10% SDS-PAGE and analysed by Western blotting. Mouse monoclonal antibodies anti-VDR (sc-13133), anti E-Cadherin (sc-21791) and goat antibody anti-UCP2 (sc-6525) were from Santa Cruz, CA, USA. Rabbit anti-UCP1 was obtained from Sigma (U6382). Our previous works [17, 18] have shown that the antibody against VDR gives some unspecific signal. The correct band was identified in past studies by molecular weight and silencing experiments in HaCaT, MCF7 and HeLa cells [17, 18] , and corresponds to the lower band when a doublet band is present. The signal that detects VDR is indicated in all figures. The loading controls were carried out on the same membranes and detected by antibodies anti-VDAC (monoclonal anti-porin 31HL, Calbiochem), anti-actin (mouse monoclonal sc-8432 Santa Cruz) and rabbit antibody anti-PARP (sc-7150, Santa Cruz).
Fifty µg of total lysates or thirty µg of nuclear or mitochondrial fractions were separated by 10% SDS-PAGE and analysed by Western blotting. Mouse monoclonal antibodies anti-VDR (sc-13133), anti E-Cadherin (sc-21791) and goat antibody anti-UCP2 (sc-6525) were from Santa Cruz, CA, USA. Rabbit anti-UCP1 was obtained from Sigma (U6382). Our previous works [17, 18] have shown that the antibody against VDR gives some unspecific signal. The correct band was identified in past studies by molecular weight and silencing experiments in HaCaT, MCF7 and HeLa cells [17, 18] , and corresponds to the lower band when a doublet band is present. The signal that detects VDR is indicated in all figures. The loading controls were carried out on the same membranes and detected by antibodies anti-VDAC (monoclonal anti-porin 31HL, Calbiochem), anti-actin (mouse monoclonal sc-8432 Santa Cruz) and rabbit antibody anti-PARP (sc-7150, Santa Cruz).
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