The release of plumbagin was studied using a dialysis method at pHs 7.4 and 5.5, respectively mimicking the physiological pH in normal tissues and blood, and the extracellular microenvironment of tumor tissues. To do so, plumbagin (500 µg/mL in phosphate buffer for 1 mL) either formulated as Tf-bearing LPN, control LPN or in solution, was transferred into a SnakeSkin® dialysis bag (molecular weight cut-off: 3 500 Daltons) (ThermoFisher Scientific, Waltham, MA) and was dialyzed against 50 mL of phosphate buffer pHs 7.4 and 5.5. The samples were kept at 37°C with continuous stirring (100 rpm). At specific time intervals (30 min, then every hour for the first 6 h (1 h, 2 h, 3 h, 4 h, 5 h, 6 h), then every 2 h for the next 6 h (8 h, 10 h, 12 h), and 24 h), 1 mL of the dialysate was withdrawn in triplicates and then replaced with an equal volume of fresh buffer. The amount of plumbagin in the sample was determined by spectrophotometry (Agilent Technologies, Santa Clara, CA) and reported as a percentage cumulative drug release.
Snakeskin dialysis bag
Manufactured by Thermo Fisher Scientific
Sourced in United States
SnakeSkin dialysis bags are a type of lab equipment used for dialysis, a process of separating molecules in a solution by diffusion across a semi-permeable membrane. The bags are made of a specialized material that allows for the selective passage of molecules based on their size, enabling the isolation and concentration of specific components in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Hvlp04700, by Merck Group (2 mentions)
Spectramax m5, by Molecular Devices (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Formic acid, by Avantor (1 mentions)
Fei tecnai g2 spirit twin transmission electron microscope, by Thermo Fisher Scientific (1 mentions)
Malvern zetasizer nano z, by Malvern Panalytical (1 mentions)
Fetal bovine serum (fbs), by Natocor (1 mentions)
Anaerobic atmosphere generation bags, by Mitsubishi (1 mentions)
Arium 611 uv, by Sartorius (1 mentions)
Dulbecco s modified eagle medium, by Merck Group (1 mentions)
Arium 61316 ro, by Sartorius (1 mentions)
7 protocols using snakeskin dialysis bag
1
Plumbagin Release Kinetics in Dialysis
2
Synthesis of Lignin Nanoparticles from Diverse Feedstocks
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poplar TGA lignin, birch and wheat BioLignin™ were used to synthesize lignin nanoparticles using the procedure described by Lievonen et al. [31 (link)], with modifications. Lignin was dissolved in THF at concentrations of 0.5, 1, 2, 5, 10 and 20 mg mL−1. The dissolved lignin was then filtered through a 0.45 µm syringe filter (GE Healthcare Whatman, Chicago, IL, USA) and introduced into a SnakeSkin dialysis bag (7 kDa MWCO; Thermo Scientific, Waltham, MA, USA), which was then immersed in a beaker with 1.5 L of ddH2O and placed on a magnetic stirrer. The water was changed every 8 h over the course of 45 h. A total of three biological replicates were assessed for each concentration. The suspension of lignin nanoparticles was removed from the dialysis bag with a serological pipette and stored at 4 °C. In order to compare the concentration of LNPs among different preparations, a 50 µL sample was mixed with 50 µL THF. The absorbance at 280 nm was measured in a SpectraMax M5 microplate reader (Molecular Devices, San Jose, CA, USA) against a set of lignin standards.
3
Antioxidant and Digestibility Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol (HPLC grade) and formic acid (puriss. p.a. for mass spectroscopy) were obtained from J. T. Baker (State of Mexico, Mexico) and Fluka (Steinheim, Germany), respectively. Commercial standard of (+) catechin were purchased from Extrasynthese (Genay, France). Chlorogenic acid, kaempferol and quercetin were purchased from Fluka (Dorset, U.K.). Filters (0.45 μm, HVLP04700) were obtained from Millipore (São Paulo, Brazil). ABTS (2,2′-azino-bis-(3-thylbenzothiazolne-6-sulfonic acid) diammonium salt), DPPH (1,1,-diphenyl-2-picrylhydrazyl radical), TPTZ (2,4,6-tripyridyl- S-triazine), Trolox (6-hydroxy-2,5,7,8-tetramethyl-chroman-2- carboxylic acid), Folin-Ciocalteu Reagent, pepsin (P-7000, from porcine stomach mucosa), pancreatin (P-1750, from porcine pancreas) and bile extract (from porcine, B-8631) were purchased from Sigma-Aldrich (Buenos Aires, Argentina). SnakeSkin dialysis bags with a molecular weight cut-off of 10 kDa and a width of 22 mm were obtained from ThermoFisher SCIENTIFIC. All other reagents were of analytical grade.
4
Purification of hERG-GFP-His Fusion Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified hERG-GFP-His 8 fusion protein were digested O/N in snakeskin dialysis bags (Thermo Scientific, USA) with dialysis buffer (20 mM phosphate pH 7.0 200 mM NaCl 0.075% (w/v) FC-12 0.026% (w/v) CHS) and TEV protease [32 ] at room temperature with a protein to TEV ratio of 1:10 (w/w). Digestion efficiency was estimated by in-gel fluorescence followed by Coomassie staining.
5
Characterization and Evaluation of C. odorata Leaf Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C. odorata leaves were identified by a qualified botanist and were collected from the Botanical Garden of Jamia Hamdard, New Delhi, India with specimen number: BOT/DAC/2023/32. The following equipment was used: a UV-VIS spectrophotometer (Shimadzu-1700, Tokyo, Japan), rotor–stator mixer (Witeg HG-15D, Baden-Württemberg, Germany), 20 kHz Sonicator (Ultrasonics, Sonics & Materials, Inc., Newtown, CT, USA), Malvern Zetasizer Nano ZS (Malvern Panalytical Ltd., Malvern, UK), Brookfield cone and plate viscometer (Brookfield Engineering Laboratories, Inc., Middleboro, MA, USA), pH meter (Hanna Edge, Providence, RI, USA), Sebumeter® SM 815 (Tokyo, Japan), Corneometer® CM 825 device (Courage & Khazaka, Electronic GmbH, Köln, Germany), SnakeSkin dialysis bags (Thermo Scientific, Maharashtra, India), and FEI Tecnai G2 Spirit Twin Transmission electron microscope (FEI Company, Uniondale, NY, USA), Minneapolis. C. odorata oil (CAS Number: 8006-81-3), Cremophor® EL (CAS Number: 61791-12-6), Di (ethylene glycol) ethyl ether (Transcutol® HP, Gattefosse Corp Company, Paramus, NJ, USA) (CAS Number: 111-90-0), and Poloxamer 188 (CAS Number: 9003-11-6) were purchased from Sigma Aldrich Chemicals Private Limited, Bangalore, India. Preserved skin samples were purchased from ETSY Pvt. Ltd., Gurgaon, India. All the solvents and chemicals were of a good analytical grade.
6
Purified AQP-TEV-GFP-His10 Cleavage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified AQP-TEV-GFP-His10 fusion proteins were digested O/N in snakeskin dialysis bags (Thermo Scientific, USA) with dialysis buffer (20 mM phosphate pH 7.0, 200 mM NaCl, 1.5 x CMC detergent) and TEV protease33 (link) at room temperature with a protein to TEV ratio of 1:10 (w/w). Digestion efficiency was estimated by in-gel fluorescence.
7
In Vitro Polyphenol Digestion and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ultra-pure water (<18 MΩ cm < 5 μgL−1 TOC) was obtained from a purification system Arium 61316-RO plus Arium 611 UV (Sartorius, Germany). Methanol (HPLC grade) and formic acid (puriss. p. a. for mass spectroscopy) were provided by J. T. Baker (State of Mexico, Mexico) and Merck (California, USA), respectively. Commercial standards of polyphenolic compounds were obtained from Extrasynthese (Genay, France), Sigma-Aldrich (Steinheim, Germany), and Fluka (Dorset, U.K.). Filters (0.45 μm, HVLP04700) were obtained from Millipore (São Paulo, Brazil). Porcine enzymes used in in vitro gastrointestinal digestion and all reagents used for redox markers in cell culture were purchased from Sigma-Aldrich (Buenos Aires, Argentina). SnakeSkin dialysis bags with a molecular weight cut-off of 10 kDa and a width of 22 mm, and Hypersep SPE 500 mg/2.8 mL C18 cartridges were obtained from ThermoFisher SCIENTIFIC. Anaerobic atmosphere generation bags were purchased from Mitsubishi Gas Chemical (Tokyo, Japan). Dulbecco's modified Eagle medium was obtained from Sigma-Aldrich and fetal bovine serum from Natocor (Córdoba, Argentina). All other reagents were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!