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5 5 dithiobis 2 nitrobenzoic acid dtnb tacrine

Manufactured by Merck Group
Sourced in United States

5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) is a chemical compound used as a reagent in various biochemical and analytical applications. Tacrine is a drug that was previously used for the treatment of Alzheimer's disease.

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2 protocols using 5 5 dithiobis 2 nitrobenzoic acid dtnb tacrine

1

Enzyme Assays for Neurodegenerative Disorders

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AChE (Type VI-S; from Electrophorus electricus), recombinant human MAO-A, MAO-B, and BChE (from equine serum), acetylthiocholine iodide (ATCI), kynuramine, benzylamine, S-butyrylthiocholine iodide (BTCI), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), tacrine, donepezil, toloxatone, and lazabemide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Clorgyline and pargyline (irreversible reference inhibitors of MAO-A and MAO-B, respectively) were from BioAssay Systems (Hayward, CA, USA)40 (link).
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2

Cholinesterase Inhibition Assay Protocol

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All HPLC solvents were purchased from Guangdong Guanghua Sci-Tech Co. Ltd. (Guangzhou, China). Acetylcholinesterase (C3389), acetylthiocholine iodide (A5751), butyrylcholinesterase (C1057), butyrylthiocholine iodide (B3253), and 5,5-dithiobis (2-nitrobenzoic acid) (DTNB), tacrine and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich Co. (St. Louis, Missouri, United States). All chemicals and solvents used in column chromatography and assays were acquired from commercial sources.
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