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2 protocols using l homopropargylglycine hpg

1

Metabolic Labeling Techniques in Cell Culture

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Collagen type 1 (rat tail) was from Millipore or from PureCol® (bovine) (Advanced Biomatrix, USA). The CPT1a inhibitor (+)-etomoxir sodium salt hydrate was purchased from CNIO Carlos III Therapies. Mitomycin C (MitoC), sodium palmitate, dimethyl sulfoxide (DMSO), NAC, sodium acetate, oligomycin, cycloheximide, cytidine, adenine, guanosine, methotrexate, carnitine, dNTP mix and tamoxifen were from Sigma-Aldrich (Bornem, Belgium). 5-fluorouracil (TEVA Pharma Belgium) was obtained from the pharmacy of the university hospital Leuven. Nucleoside mix was from Millipore (Belgium)15 . Hoechst 33342 and L-homopropargylglycine (HPG) were from Molecular Probes and L-glutamine and penicillin/streptomycin were from Gibco® (Invitrogen, Life Technologies, Ghent, Belgium). Uniformly labeled [U-13C]-potassium palmitate, [U-13C]-acetate, [U-13C]-glucose, [U-13C]-glutamine and [U-13C]-algal fatty acid mix were obtained from Cambridge isotope laboratories, Inc. [U-14C]-palmitate, [9,10-3H]-palmitate, [6-14C]-D-glucose, [8-14C]-hypoxanthine and [3H]-thymidine were from Perkin Elmer.
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2

Metabolic Labeling Techniques in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Collagen type 1 (rat tail) was from Millipore or from PureCol® (bovine) (Advanced Biomatrix, USA). The CPT1a inhibitor (+)-etomoxir sodium salt hydrate was purchased from CNIO Carlos III Therapies. Mitomycin C (MitoC), sodium palmitate, dimethyl sulfoxide (DMSO), NAC, sodium acetate, oligomycin, cycloheximide, cytidine, adenine, guanosine, methotrexate, carnitine, dNTP mix and tamoxifen were from Sigma-Aldrich (Bornem, Belgium). 5-fluorouracil (TEVA Pharma Belgium) was obtained from the pharmacy of the university hospital Leuven. Nucleoside mix was from Millipore (Belgium)15 . Hoechst 33342 and L-homopropargylglycine (HPG) were from Molecular Probes and L-glutamine and penicillin/streptomycin were from Gibco® (Invitrogen, Life Technologies, Ghent, Belgium). Uniformly labeled [U-13C]-potassium palmitate, [U-13C]-acetate, [U-13C]-glucose, [U-13C]-glutamine and [U-13C]-algal fatty acid mix were obtained from Cambridge isotope laboratories, Inc. [U-14C]-palmitate, [9,10-3H]-palmitate, [6-14C]-D-glucose, [8-14C]-hypoxanthine and [3H]-thymidine were from Perkin Elmer.
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