Enhanced chemiluminescence ecl plus kit

The 2-Chloroethanol was purchased from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China). Reagents for the primary culture of astrocytes were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative real-time (RT)-PCR assay kit was purchased from Takara, Japan. The enhanced chemiluminescence (ECL) plus kit, bicinchoninic acid (BCA) protein assay kit and NE-PER™ nuclear and cytoplasmic extraction reagents were obtained from Thermo Fisher Scientific (Waltham, MA, USA). SB202190, pyrrolidine dithiocarbamate (PDTC), and SR11302 were purchased from Selleck (Houston, TX, USA) and APExBIO (Houston, TX, USA). Primary antibodies against MMP-9, p65, IκBα, c-Jun, c-Fos, p-c-Jun, p-c-Fos, p-IκBα, and LaminB were products of Abcam (Cambridge, UK) and Cell Signaling Technology (Beverly, MA, USA). Antibodies against glial fibrillary acid protein (GFAP), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and β-actin were obtained from Millipore (Billerica, MA, USA), Proteintech (Wuhan, China), and ABclonal (Wuhan, China), respectively. The secondary antibodies conjugated with Alexa Fluor 488 or tetramethylrhodamine (TRITC), RIPA Lysis Buffer, and DAPI were obtained from Beyotime Biotechnology (Shanghai, China).

Antibodies against CYP2E1 (#AB1252), occludin (#40-4700), heme oxygenase-1 (HO-1, A1346) and β-actin were purchased from Millipore (United States), Thermo Fisher Scientific (United States), ABclonal (China), and Santa Cruz Biotech (United States), respectively. Antibodies against claudin-5 (#ab15106) and nuclear factor erythroid 2-related factor 2 (Nrf2, #ab62352) were the products of Abcam (Cambridge, MA, United States). Horseradish peroxidase was obtained from ZSGB Biotechnology (China). The assay kits for detecting superoxide dismutase (SOD) activity and the levels of malondialdehyde (MDA), non-protein sulfhydryl (NPSH) and creatinine were purchased from Nanjing Jiancheng Bioengineering Co. Ltd (China). DAS was the product of Tokyo Chemical Industry (Japan). A bicinchoninic acid (BCA) protein assay kit and an enhanced chemiluminescence (ECL) plus kit were the products of Thermo Fisher Scientific (United States). RIPA Lysis Buffer was the products of Beyotime Biotechnology (China). TRIzol reagent was purchased from Takara (Japan). All other chemicals used were analytical grade and were purchased from local chemical suppliers. Double-distilled water was used in this study.