Orbital shaker

Manufactured by Benchmark Scientific

Sourced in United States

The Orbital Shaker is a laboratory equipment designed to provide gentle, uniform mixing and agitation for a variety of applications. The core function of the Orbital Shaker is to create a circular motion to mix or suspend liquids, cells, or other samples within a container, such as a flask or a petri dish.

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Lab products found in correlation

Uv visible spectroscopy, by Shimadzu (1 mentions)

3 protocols using orbital shaker

Isolation and Purification of Microglia

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Upon confluence, loosely adherent cells were dislodged from culture flasks by agitation using an orbital shaker (Benchmark, Edison, NJ, USA). Flasks were shaken at 260 rpm for 4 h and supernatants were harvested and centrifuged. Before the start of the in vitro co-culture experiments, cells were resuspended in culture medium free of GM-CSF, counted, and seeded into 24 well plates at a density of 2×10⁵ cells per well and incubated at 37 °C and 5% CO₂ for 5 days prior to assay. Remaining cells were evaluated for purity and their phenotype was determined by flow cytometry. CD11b⁺CD45⁺ microglia comprised ≥95% of cells harvested in this manner.

Benedek G., Zhang J., Nguyen H., Kent G., Seifert H., Vandenbark A.A, & Offner H. (2017). Novel feedback loop between M2 macrophages/microglia and regulatory B cells in estrogen-protected EAE mice. Journal of neuroimmunology, 305, 59-67.

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Isolation of Microglia from Cell Cultures

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Upon confluence, loosely adherent cells were dislodged from culture flasks by agitation using an orbital shaker (Benchmark, Edison, NJ, USA). Flasks were shaken at 280 revolutions per minute for 4 hours and supernatants were harvested and centrifuged. Before the start of the in vitro co-culture experiments, cells were resuspended in culture medium free of GM-CSF, counted, and seeded into 24 well plates at a density of 2×10⁵ cells per well and incubated at 37°C and 5% CO₂ for 5 days prior to assay. Remaining cells were checked for purity and their phenotype was determined by flow cytometry. CD11b⁺CD45⁺ microglia comprised ≥96% of cells harvested in this manner.

Bodhankar S., Lapato A., Chen Y., Vandenbark A.A., Saugstad J.A, & Offner H. (2015). Role for Microglia in Sex Differences after ischemic stroke: Importance of M2. Metabolic brain disease, 30(6), 1515-1529.

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Evaluating Crosslinked Vancomycin-Loaded Gelatin Beads

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To evaluate the crosslinking level of the gelatin, the beads were validated by Ninhydrin assay (Sigma-Alderich, St. Louis, MO, USA) by a 100 °C water bath for 20 min. The free amine amount was evaluated by 570 nm absorption using UV/visible spectroscopy (Shimadzu, Kyoto, Japan). The drug loading capacity was evaluated by grinding weighted vancomycin-loaded gelatin bead samples. Drug releasing was achieved by the continuous shaking of bead debris in 400 mL of PBS using an orbital shaker (Benchmark Scientific, Sayreville, NJ, USA) for 2 h. The supernatant was collected and evaluated by 280 nm absorption using UV/visible spectroscopy (Shimadzu, Kyoto, Japan). The evaluation of drug releasing kinetics follows a similar process without grinding. Similar weight vancomycin-loaded gelatin bead samples were incubated in the 96-well culture plates with 200 μL of PBS added in a 37 °C incubator for monitoring the in vivo environment and evaluated by spectroscopy by fixed time points.

Wang Z., Yang S., He C., Li C, & Louh R.F. (2024). Enhancing Bone Cement Efficacy with Hydrogel Beads Synthesized by Droplet Microfluidics. Nanomaterials, 14(3), 302.

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