Ft ir 5300 spectrophotometer

Manufactured by Jasco

Sourced in Italy

The FT-IR 5300 spectrophotometer is a laboratory instrument designed for infrared spectroscopy analysis. It is capable of measuring the absorption or transmission of infrared radiation by a sample, providing information about the chemical composition and molecular structure of the material.

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Lab products found in correlation

Jms t100lc spectrometer, by JEOL (2 mentions) Avance 2 series 200 mhz spectrometer, by Bruker (1 mentions) Precoated silica plates g f254, by Merck Group (1 mentions) Malvern zetasize nano zs90, by Malvern Panalytical (1 mentions) Jsm 6510 v, by JEOL (1 mentions) Atm 01, by As One (1 mentions) Hplc system, by Shimadzu (1 mentions) Ecx 500 spectrometer, by JEOL (1 mentions) Tottoli capillary apparatus, by Büchi (1 mentions) Amx 500 spectrometer, by Agilent Technologies (1 mentions) Dip 370 polarimeter, by Jasco (1 mentions)

10 protocols using ft ir 5300 spectrophotometer

Synthesis of 1-Substituted 1,5,6,7-Tetrahydro-4H-Indazol-4-Ones

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All melting points were taken on a Buchi-Tottoli capillary apparatus; IR spectra were determined, in CHBr 3 , with a Jasco FT/IR 5300 spectrophotometer; 1 H and 13 C NMR spectra were measured in DMSO-d 6 or CDCl 3 solutions, at 200 and 50 MHz respectively, using a Bruker Avance II series 200 MHz spectrometer. Column chromatography was performed with Merck silica gel 230e400 Mesh ASTM or with a SEPACOR chromatography apparatus BÜCHI. Elemental analyses (C, H, N) were within ±0.4% of the theoretical values.
Compound 7 was prepared according to procedure described elsewhere [39] .
5.1.1. General procedure for the preparation of 1-substituted 1,5,6,7tetrahydro-4H-indazol-4-ones(8a-h)
To a solution of compound 7 (3 g, 18 mmol) in a mixture of ethanol and acetic acid (2:1) (48 mL) or, for the synthesis of compounds 8 e-g, only glacial acetic acid (30 mL) the suitable hydrazine (18 mmol) was added and the reaction mixture was heated under reflux for 2 h. Then, the reaction mixture was poured onto crushed ice and the solid was filtered off, dried and purified by chromatography (DCM/AcOEt 9:1) as eluent.
5.1.1.1. 1,5,6,7-Tetrahydro-4H-indazol-4-one (8a). This compound was obtained from reaction of 7 with hydrazine hydrate. Yellow solid; yield: 67%; m.p. 165e166 C; IR cm À1 : 3186 (NH), 1647 (CO);

Spanò V., Parrino B., Carbone A., Montalbano A., Salvador A., Brun P., Vedaldi D., Diana P., Cirrincione G, & Barraja P. (2015). Pyrazolo[3,4-h]quinolines promising photosensitizing agents in the treatment of cancer. European journal of medicinal chemistry, 102.

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Synthesis of Benzotriazole Analogues

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Synthesis was carried out using modified reported procedures for conventional synthesis[13 14 15 16 17 (link)] and reactions were modified to improve the yield and purity of products. For comparison, microwave-assisted synthesis was also carried out. All solvents and reagents used for synthesis were of general reagent grade. The reactions were monitored by thin layer chromatography (TLC) with Merck precoated silica plates (GF 254). Melting points were recorded in open capillaries in a thiel's tube melting point apparatus and are uncorrected. Infrared (IR) was recorded (KBr disc method) on a Jasco Fourier transform infrared spectroscopy FT-IR 5300 spectrophotometer. ¹H NMR spectra were recorded on Varian USA Mercury Plus (300 MHz) or Bruker (300 MHz) NMR spectrophotometer. Chemical shifts are reported in ppm down field from tetramethylsilane (TMS), which is used as the internal standard. MW-assisted reactions were carried out in a domestic microwave oven (Samsung M183DN) at 180 and 300 W.
General synthetic scheme was used to synthesise 1 and 5 substituted benzotriazole analogues as shown in figs. 1–3.

Shah J.J, & Mohanraj K. (2014). Comparison of Conventional and Microwave-assisted Synthesis of Benzotriazole Derivatives. Indian Journal of Pharmaceutical Sciences, 76(1), 46-53.

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Characterization of ZnO/Alg-NCMs Nanoparticles

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The characterization of ZnO/Alg-NCMs was performed by Fourier transform infrared spectroscopy (FTIR) using a (JASCO Europe S.r.l., Cremella, Italy), FT-IR 5300 spectrophotometer. Morphology and particle size of the nanoparticles were characterized via scanning electron microscopy JEOL JSM-6510/v, Tokyo, Japan (SEM), and transmission electron microscopy (JEOL JSM-6510/v, Tokyo, Japan), (TEM). The chemical contents of the nanoparticles were scanned by energy dispersive X-ray spectroscopy (EDS) using a Zeta potential analyser (Malvern Zeta size Nano-Zs90) Malvern United States.

Hamouda R.A., Salman A.S., Alharbi A.A., Alhasani R.H, & Elshamy M.M. (2021). Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga Fucus vesiculosus in Mice. Polymers, 13(21), 3839.

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Fungal Metabolites Characterization by FT-IR

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The ethyl acetate extracts of fungal secondary metabolites and the purified sample was dried, powdered and pelleted for FT-IR analysis (Jasco FTIR 5300 spectrophotometer). The sample (1.0 mg) was mixed with 100 mg of KBr (binding agent) using a clean mortar and pestle to make the powder into tablet [47 (link)]. The changes in the surface chemical bonding and surface composition was characterized using Fourier Transform Infrared (FT-IR) spectroscopy (Nicolet Avatar series 330) ranged from 400 to 4,000 cm⁻¹. Measurements were carried out to identify the possible bio-molecules responsible for dead mosquito larvae by fungal ethyl acetate extract [64 , 65 ].

Baskar K., Chinnasamy R., Pandy K., Venkatesan M., Sebastian P.J., Subban M., Thomas A., Kweka E.J, & Devarajan N. (2020). Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus. Heliyon, 6(10), e05331.

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Synthesis and Characterization of Organic Compounds

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All reactions were carried out in oven-dried glassware under an argon atmosphere. Flash column chromatography was performed with silica gel Merck 60 (230–400 mesh ASTM, Darmstadt, Germany). TLC analysis was performed on 0.25 mm Silicagel Merck 60 F₂₅₄ plates. Melting points were determined on a hot stage microscope apparatus (AS ONE, ATM-01, Osaka, Japan and were uncorrected. NMR spectra were recorded on a JEOL DELTA 300 (Tokyo, Japan) or JEOLRESONANCE ECX-500 spectrometer (Tokyo, Japan), operating at 300 MHz or 500 MHz for ¹H-NMR and 75 MHz 120 MHz for ¹³C-NMR. Chemical shifts (δ ppm) in CDCl₃ were reported downfield from TMS (= 0) for ¹H-NMR. For ¹³C-NMR, chemical shifts were reported in the scale relative to CDCl₃ (77.00 ppm) as an internal reference.IR Spectra were recorded on a JASCO FT/IR-5300 spectrophotometer (Tokyo, Japan). Mass spectra were measured on a JEOL JMS-T100LC spectrometer (Tokyo, Japan). HPLC data were obtained on a SHIMADZU HPLC system (consisting of the following: LC-20AT, CMB20A, CTO-20AC, and detector SPD-20A measured at 254 nm, Kyoto, Japan) using Daicel Chiracel AD-H or Ad-3 column (25 cm) at 25 °C. Optical rotations were measured on a JASCO DIP-370 (Na lamp, 589 nm, Tokyo, Japan).

Fujiwara T., Tsutsumi T., Nakata K., Nakatsuji H, & Tanabe Y. (2017). Asymmetric Total Syntheses of Two 3-Acyl-5,6-dihydro-2H-pyrones: (R)-Podoblastin-S and (R)-Lachnelluloic Acid with Verification of the Absolute Configuration of (−)-Lachnelluloic Acid. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(1), 69.

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Characterization of Fu/ZnO-NPs and Fu/Alg-ZnO-NCMs

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The Fu/ZnO-NPs and Fu/Alg-ZnO-NCMs active groups were assessed using a Fourier transform infrared spectrometer (FT-IR) (JASCO Europe S.r.l., Cremella, Italy), FT-IR 5300 spectrophotometer; the FT-IR spectrum ranged between 4000 and 400 cm⁻¹.

Hamouda R.A., Alharbi A.A., Al-Tuwaijri M.M, & Makharita R.R. (2023). The Antibacterial Activities and Characterizations of Biosynthesized Zinc Oxide Nanoparticles, and Their Coated with Alginate Derived from Fucus vesiculosus. Polymers, 15(10), 2335.

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Synthesis and Characterization of Novel Molecules

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The molecules (Table 1) were synthesised by established procedures[13 14 ] as shown in Schemes 1 and 2. The procedures were modified to improve the yield and purity of the products. All solvents and reagents used for synthesis were of laboratory grade. The reactions were monitored by thin layer chromatography (TLC) using Merck precoated silica plates (GF 254) for completion of the reaction and for establishing the purity. The melting points were recorded in open capillaries on an electrically heated melting point apparatus and are uncorrected. IR spectra were recorded (KBr disc method) on a Jasco FT-IR 5300 spectrophotometer. ¹H NMR spectra for some representative structures were recorded on a 300 MHz Bruker AN 300 instrument. Chemical shifts are reported in ppm down field from tetramethylsilane (TMS) as the internal standard. Mass spectra were also carried out for some representative compounds. Spectroscopic details for representative molecules are provided in the Table 2.

Shaikh M.S., Rana J., Gaikwad D., Leartsakulpanich U., Ambre P.K., Pissurlenkar R.R, & Coutinho E.C. (2014). Antifolate Agents Against Wild and Mutant Strains of Plasmodium falciparum. Indian Journal of Pharmaceutical Sciences, 76(2), 116-124.

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Detailed Spectroscopic Analysis of Organic Compounds

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Unless otherwise indicated, all reagents and solvents were purchased from commercial sources and used without further purification. All melting points (°C) were determined on a Tottoli capillary apparatus (Büchi) and are uncorrected; IR spectra were determined in bromoform with a FT/IR 5300 spectrophotometer (Jasco). ¹H-NMR and ¹³C-NMR spectra were respectively recorded, at 200 and 50.3 MHz in CDCl₃ or DMSO-d₆ solution, using an AC-E series 200 MHz spectrometer (Bruker). Chemical shifts values are given in ppm and referred as the internal standard to tetramethylsilane (TMS). The following abbreviations are used: br s = broad signal, s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, rt = room temperature. The purity of all compounds screened in biological assays was determined to be >95% by HPLC/MS analysis. Thin layer chromatography was performed on precoated (0.25 mm) silica gel GF254 plates, compounds were detected with 254 nm UV lamp. Column chromatography was performed with silica gel ASTM (230 and 400 mesh, Merck), or with a FLASH40i chromatography module (prepacked cartridge system, Biotage).

Lauria A., La Monica G., Terenzi A., Mannino G., Bonsignore R., Bono A., Almerico A.M., Barone G., Gentile C, & Martorana A. (2021). Antiproliferative Properties and G-Quadruplex-Binding of Symmetrical Naphtho[1,2-b:8,7-b’]dithiophene Derivatives. Molecules, 26(14), 4309.

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FT-IR Spectroscopy of Inclusion Complexes

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The FT-IR spectra of BSM, β-CD, 2-HP-β-CD, their physical mixtures, and the inclusion complexes of BSM/β-CD and BSM/2-HP-β-CD were obtained by a Jasco FT-IR 5300 spectrophotometer using KBr pellets, and the wavelength ranging from 4000 cm⁻¹ to 450 cm⁻¹ with a resolution of 0.5 cm⁻¹.

Geng Q., Li T., Wang X., Chu W., Cai M., Xie J, & Ni H. (2019). The mechanism of bensulfuron-methyl complexation with β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin and effect on soil adsorption and bio-activity. Scientific Reports, 9, 1882.

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Isolation and Characterization of Amphidinium Species

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General Experimental Procedures Optical rotation and IR data were measured on a JASCO DIP-370 polarimeter and a JASCO FT/IR-5300 spectrophotometer, respectively. NMR data were recorded using 2.5 mm microcells (Shigemi Co., Ltd., Japan). NMR spectra were measured on a Bruker AMX-500 spectrometer or a Varian-NMR500 spectrometer equipped with a triple resonance PFG Cold Probe or CH "Xsens" PFG Cold Probe. Chemical shifts were reported in ppm with reference to the residual proton and carbon signals of C 6 D 6 (δ H 7.20 and δ C 128.0, respectively) and CDCl 3 (δ H 7.26 and δ C 77.0, respectively). ESI-MS spectra were recorded on a JEOL JMS-T100LC spectrometer.
Materials A dinoflagellate Amphidinium species (strain KCA09053) was monoclonally separated from benthic sea sands collected off Iriomote Island, Japan in May 2009. 6) (link) The voucher specimen was deposited at the Center for Advanced Marine Core Research, Kochi University.

Akakabe M., Kumagai K., Tsuda M., Konishi Y., Tominaga A., Kaneno D., Fukushi E., Kawabata J., Masuda A, & Tsuda M. (2016). Iriomoteolides-10a and 12a, Cytotoxic Macrolides from Marine Dinoflagellate Amphidinium Species. Chemical & pharmaceutical bulletin, 64(7).

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