Arid1b

Protein lysates were quantified using the Micro BCA Protein Assay Kit (ThermoFisher) and a FlexSystem3 plate reader. Protein lysates were run on a 4–15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel (BioRad) and transferred to PVDF membrane using the TransBlot Turbo system (BioRad). Primary antibodies were used at the following dilutions: 1:1000 ARID1A (D2A8U) (12354, Cell Signaling); 1:1000 Akt (4691, Cell Signaling); 1:1000 β-Actin (8457, Cell Signaling); E-Cadherin (3195, Cell Signaling); 1:2000 Phospho-Akt (Ser473) (4060, Cell Signaling); 1:1000 Slug (9585, Cell Signaling); 1:1000 Snail (3879, Cell Signaling); 1:1000 Twist1 (T6451, Sigma); 1:100 ARID1B (sc-32762, Santa Cruz); 1:1000 Brg1 (ab110641, Abcam); 1:1000 BRM (11966, Cell Signaling); 1:100 ARID1A (PSG3) (sc-32761, Santa Cruz). Horseradish peroxidase (HRP) conjugated secondary antibodies (Cell Signaling) were used at a dilution of 1:2000. Clarity Western ECL Substrate (BioRad) was used for protein band visualization, and western blot exposures were captured using the ChemiDoc XRS + imaging system (BioRad). Uncropped western blot images can be found in Supplementary Fig. 7.