For cell proliferation assays, 2.5×104 cells were seeded into tissue-culture treated adherent 6-well plates (Falcon). Cells were collected through trypsin digestion and counted on days 1 and 5. Cell counts were normalized to day 1. For colony formation assays, 1×102 cells were seeded in triplicate into tissue-culture treated adherent 10cm plates (Falcon). On day 14, plates were washed with PBS, fixed in 6% glutaraldehyde (Sigma-Aldrich), stained with 0.5% crystal violet (Sigma-Aldrich), and washed with H20 to better enable the visualization of colonies.
Tissue culture treated adherent 6 well plates
Manufactured by BD
Tissue-culture treated adherent 6-well plates are a type of laboratory equipment designed for cell culture applications. These plates provide a surface that promotes the attachment and growth of a variety of cell types. The plates have six individual wells, each with a surface area optimized for cell culture.
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2 protocols using tissue culture treated adherent 6 well plates
1
Cell Proliferation and Colony Formation Assays
2
Cell Proliferation and Colony Formation Assays
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For cell proliferation assays, 2.5×104 cells were seeded into tissue-culture treated adherent 6-well plates (Falcon). Cells were collected through trypsin digestion and counted on days 1 and 5. Cell counts were normalized to day 1. For colony formation assays, 1×102 cells were seeded in triplicate into tissue-culture treated adherent 10cm plates (Falcon). On day 14, plates were washed with PBS, fixed in 6% glutaraldehyde (Sigma-Aldrich), stained with 0.5% crystal violet (Sigma-Aldrich), and washed with H20 to better enable the visualization of colonies.
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