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Anti atm d2e2

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Anti-ATM(D2E2) is a monoclonal antibody that recognizes the ATM (Ataxia Telangiectasia Mutated) protein. ATM is a serine/threonine protein kinase that plays a critical role in the cellular response to DNA double-strand breaks and other forms of DNA damage.

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Lab products found in correlation

Flag m2 affinity gel, by Merck Group (1 mentions) Anti phospho atm ser1981, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Etoposide, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti flag m2, by Merck Group (1 mentions) Subcellular protein fractionation kit for cultured cells, by Thermo Fisher Scientific (1 mentions) Anti ph2ax ser139, by Merck Group (1 mentions) Anti h2ax, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti parp, by Cell Signaling Technology (1 mentions)

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ATM (D2E2) (3 citations)

2 protocols using anti atm d2e2

1

Immunoblotting of DNA Damage Proteins

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Immunoblotting was performed using antibodies as indicated: anti-Cullin1 (D-5, sc-17775, Santa Cruz), anti-Skp1 (H-6, sc-5281, Santa Cruz), anti-Chk1 (2G11D5, sc-56288, Santa Cruz), anti-GAPDH (G-9, sc-365062, Santa Cruz), anti-beta-TcrP (4394, Cell Signaling), anti-hSSB1 (ab85752, Abcam), anti-FBXL5 (ab68069, Abcam), anti-FBXO6 (C-20, sc-323856, Santa Cruz), anti-Skp2 (H-435, sc-7164, Santa Cruz), anti-Phospho-ATM(Ser1981) (D25E5, Cell Signaling), anti-ATM(D2E2, Cell Signaling), anti-Flag M2 (F1804, Sigma), anti-HA (3724, Cell Signaling), anti-HA (2365, Cell Signaling). FLAG M2 Affinity Gel (A2220) was purchased from Sigma. MLN4924 was purchased from Medkoo. Caffeine, etoposide and cycloheximide (CHX) were purchased from Sigma.
Chen Z.W., Liu B., Tang N.W., Xu Y.H., Ye X.Y., Li Z.M., Niu X.M., Shen S.P., Lu S, & Xu L. (2014). FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Research, 42(18), 11560-11569.
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2

PARP Trapping Effect Analysis

Cited 1 time
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Immunoblot analysis was performed as described previously (16 (link)). For studying the PARP trapping effect of the PARPi, isolation of the nuclear soluble and chromatin bound fraction was performed using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Fisher Scientific 78840) according to the manufacturer's protocol. Cells were therefore treated with 0.005% Methyl methane sulfonate (MMS, Thermo Fisher Scientific, 156890050) and 2 μmol/L talazoparib. The following primary antibodies were used for immunoblot analysis: anti-p21 Waf1/Cip1 (DCS60; Cell Signaling Technology 2946, 1:1,000), anti-pH2AX (Ser139; Merck, JBW301, 1:500), anti-β-Actin (Cell Signaling Technology 4967, 1:2,000), anti-p53 (DOI; sc-126, 1:500), anti-ATM (D2E2; Cell Signaling Technology 2873, 1:1,000), anti-PARP (Cell Signaling Technology 9542, 1:1,000), and anti-H2A.X (Cell Signaling Technology 2595, 1:1,000). The following secondary antibodies were used: anti-mouse IgG, horseradish peroxidase (HRP)-linked (Cell Signaling Technology 7076) and anti-rabbit IgG, HRP-linked (Cell Signaling Technology 7074).
Fröhlich L.M., Niessner H., Sauer B., Kämereit S., Chatziioannou E., Riel S., Sinnberg T, & Schittek B. (2023). PARP Inhibitors Effectively Reduce MAPK Inhibitor Resistant Melanoma Cell Growth and Synergize with MAPK Inhibitors through a Synthetic Lethal Interaction In Vitro and In Vivo. Cancer Research Communications, 3(9), 1743-1755.
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