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Sightx viewer software

Manufactured by JEOL
Sourced in Japan, United States

The SightX Viewer software is a tool designed to visualize and analyze data acquired with JEOL's scientific instruments. The software provides a user-friendly interface for displaying and manipulating images, spectra, and other data generated by JEOL's laboratory equipment.

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3 protocols using sightx viewer software

1

Ultrastructural Analysis of OSCs

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OSCs were fixed with ITO-fixing solution (25% paraformaldehyde, 25% Glutardialdehyde, 0.1 M cacodylatbuffer) and pre-embedded horizontally in HistoGel. The OSCs were then processed for Epon embedding following standard protocols. Ultrathin cross sections were cut with a microtome (Ultracut Leica, Jena, Germany) and analyzed with a JEM-1400Plus (JEOL, Tokyo, Japan). SightX-Viewer software (Jeol, version 1.2.3.537) was used to evaluate the morphological changes.
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2

Measuring Autophagic Compartment Sizes

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The diameter of individual autophagic compartments (autophagosomes and autolysosomes) was determined in mRFP-EGFP-LC3-expressing cells, imaged by fluorescence microscopy (SIM and confocal microscopy). Specifically, the full width at half maximum of the red fluorescence (mRFP) signal intensity was measured using ZEN microscope software (Zeiss, Oberkochen, Germany). All vesicle diameter values represent an average of two independent measurements made in the equatorial plane in two perpendicular directions (horizontally and vertically) to improve the accuracy of the measurement. We measured the diameter of all autophagic compartments that were in focus in the equatorial z stack of individual imaged cells.
The size of the autophagic compartments was also determined on TEM micrographs. Autophagy-related structures were identified based on specific morphological characteristics, such as spherical structures with a double-layered membrane containing different intercellular material, and their diameter was measured using SightX Viewer software (JEOL, Tokyo, Japan).
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3

Visualizing Nanoparticle Aggregation

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A 250 μM solution of 6 and 8 in 20 mM phosphate buffer ( pH 6.5), containing 150 mM NaCl was placed on Formvar-carboncoated 400-mesh copper grids (Electron Microscopy Sciences, Washington, PA) and stained negatively with uranyl acetate. The aggregates were characterized by TEM on a JEOL JEM-1400 transmission electron microscope (JEOL USA Inc., MA, USA) operating at 120 kV. The images were taken with an EM-15300SXV system and processed with the SightX Viewer software (also JEOL) routinely at a magnification of ×25 000.
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