A frozen kidney tissue of rats in each group was taken to extract proteins and estimate the protein by bicinchoninic acid assay (BCA) colorimetric method. The protein was denatured and separated by SDS-PAGE electrophoretic technique and transferred to a polyvinylidene difluoride (PVDF) membrane. After formulating with 5% bovine serum albumin (BSA) for 2 h, the blocked membranes were incubated with ERK1/2 (cell signaling), TGF-β1, MMP-2, MMP-7, MMP-8, MMP-11, MMP-14, TIMP1, and TIMP2 (Boster Biological Technology, Ltd., Wuhan, PR China) antibody, and then washed with tris buffered saline with Tween (TBST), and detected with a secondary antibodies (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD), and finally these membranes were subjected to chemiluminescence detection assay.
Mmp 7
Manufactured by Boster Bio
MMP-7 is a laboratory equipment product that is used to measure the level of Matrix Metalloproteinase-7 (MMP-7) in samples. MMP-7 is an enzyme that plays a role in the breakdown of extracellular matrix proteins. This product provides a tool for researchers to quantify MMP-7 levels in their studies.
Automatically generated - may contain errors
2 protocols using mmp 7
1
Protein Expression Analysis in Rat Kidney
2
Quantifying Serum MMP7 and MMP9 in LADC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sera were obtained from all LADC patients. Serum concentrations of MMP7 and MMP9 were quantified by ELISA using the MMP7, MMP9 ELISA kit (Boster, People’s Republic of China), respectively. ELISA was performed following the manufacturer’s instructions. Protein concentrations were determined by comparing the relative absorbance of the samples with known standards. All samples were examined in triplicate, and the mean values were used in subsequent statistical analyses.
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