Elx 800 iu automated microplate reader

The concentration of protein in the cultured cells was measured using an ELISA human immunoassay kit: Human Bcl-2 ELISA Kit (Elabscience, Wuhan, China, catalog no. E-EL-H0114) and Quantikine ELISA Human BDNF Immunoassay (R&D Systems, McKinley Place, MN, USA catalog no. DBD00). The absorbance was read at 450 nm using an ELX 800 IU automated Microplate Reader (Bio-Tek Instruments, Inc., Winooski, VT, USA; Gene 5 Software V 3.02). The results were analyzed using a quadratic log–log curve fit.

The procedure used to perform the assay was previously published [13 (link)]. The neurotransmitter levels were obtained and quantified using an ELISA (enzyme-linked immunosorbent assay) kit (#CEA900Ge; Cloud-Clone Corp.©, W. Fernhurst Dr., Katy, TX, USA) according to the manufacturer’s instructions. An ELX 800 IU automated Microplate Reader (Bio-Tek Instruments; Gene 5 Software V 3.02) was used to read the absorbance at 450 nm. A quadratic log-log curve fit was used to analyze the results. The supernatants (low and high K⁺ = before and after the GABA release) were analyzed for each well. In order to minimize the effect of the differences in the number of cells in the wells, the amount of GABA that had been produced per well was calculated based on the difference between the amount of GABA in a high K⁺ supernatant and the amount of GABA in a low K⁺ supernatant.

A commercially available ELISA human immunoassay kit: Human/Mouse Total Bcl-XL DuoSet IC ELISA (R&D Systems, McKinley Place, MN, USA; DYC894-2) and Human Free BDNF Quantikine ELISA (R&D Systems, DBD00) were used to measure the concentration of an overexpressed protein in the cells that had been cultured. An ELX 800 IU automated Microplate Reader (Bio-Tek Instruments, Inc., Winooski, VT, USA; Gene 5 Software V 3.02) was used to read the absorbance at 450 nm after which a quadratic log–log curve fit was used to analyze the results.