Tissue sections were treated with 3% hydrogen peroxide for 30 min to block endogenous peroxidase activity. Slides were then incubated at 4°C overnight with rabbit anti-mouse CD31 (#77699, Cell Signaling Technology, Danvers, MA USA) at 1:100 dilution. Signal was detected with Vectastain ABC Elite Kit (Vector Laboratories, Burlingame, CA, USA) and developed with diaminobenzidine substrate. Slides were counterstained with hematoxylin.
Rabbit anti mouse cd31
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-mouse CD31 is a primary antibody that binds to the CD31 (also known as PECAM-1) protein, which is expressed on the surface of endothelial cells. This antibody can be used to detect and localize CD31 in mouse tissue samples.
Automatically generated - may contain errors
2 protocols using rabbit anti mouse cd31
1
Immunohistochemical Analysis of CD31
2
Histological Assessment of Cardiac Remodeling
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Formalin‐fixed, paraffin‐embedded mid‐ventricular sections were stained with periodic acid–Schiff for cardiomyocyte cross‐sectional area (CSA), Masson's trichrome for fibrosis, and rabbit‐anti‐mouse CD31 (1:50, Cell Signaling Technologies, #77699) for capillary density. Cardiomyocyte CSAs were measured in three to five random sections (40–60 cells/section, ~200 cells/heart), which were averaged to represent a single data point for each heart. Capillary density was quantified by dividing the number of CD31+ cells by the area of randomly selected sections. Three to five sections were measured per heart and averaged to represent a single data point. Given the extensive variability in fibrosis distribution throughout the heart, BZ‐X Analyzer software (Keyence) was used to quantify fibrosis in full mid‐ventricular sections. Percent fibrosis was calculated as the ratio of fibrotic area to total tissue area. Measurements from two sections were averaged to represent a single data point for each heart. Quantitative histologic analyses were done in a blinded fashion.
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