Vitros 350 chemistry systems analyzer

Urinary specific gravity was determined with dipstick analyses of the urine samples, using Bayer Clinitek Chemistry Analyzer with Multistix 10SG reagent strips (Siemens Diagnostics, United States). The blood samples were centrifuged at 3500 RPM for approximately 10 min to separate the serum from red blood cells. Samples were analyzed within a few hours of collection. The creatinine (Cr) and uric acid in serum samples were analyzed using a single-slide enzymatic method, the VITROS CREA and the URIC slide method with the VITROS Calibrator Kit 1, which uses three levels of calibration to obtain quantitative measurements read by the automated VITROS 350 Chemistry Systems analyzer (Ortho Clinical Diagnostics, Rochester, NY). The serum osmolality (mOsm/L) was calculated from the sodium, blood urea nitrogen (BUN), and glucose in serum, which were measured using an enzymatic method with a fully automated VITROS 350 chemistry system analyzer (Vitros Na, BUN, GLUC; Ortho Clinical Diagnostics, Rochester, NY). After the laboratory analyses, remaining urine was transferred to 15 mL polyethylene tubes and remaining blood serum was transferred to cryogenic vials and transported on ice to be stored at −80 °C at the University of Arizona.