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Lamin a ab26300

Manufactured by Abcam
Sourced in United States

Lamin-A (ab26300) is a primary structural component of the nuclear lamina, a protein meshwork associated with the inner nuclear membrane. It is involved in nuclear assembly, chromatin organization, and regulation of gene expression.

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2 protocols using lamin a ab26300

1

Molecular Mechanisms of Neuroprotection

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Tert-buthylhydroquinone (tBHQ), 3-nitropropionic acid (3-NP) and the rest of chemicals and reagents were purchased from Sigma Chemical Co. (St. Louis, MO). Antibodies were obtained from different sources as follows: Nrf2 (sc-722), NFκB p65 (sc-8008), NFκB p50 (sc-8414), glutathione S-transferase (GST, sc-138), superoxide dismutase (SOD-1, sc-271014), tubulin-β (sc-58888), and secondary rabbit and mouse antibodies conjugated with horseradish peroxidase IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Heme-oxigenase-1 (HO-1, #3391) from BioVision (Milpitas, CA), Lamin-A (ab26300) and GFAP (ab7260) from Abcam (Cambridge, MA). Chemiluminescent detection system and FluoroJade B (AG3010) were obtained from Millipore (Bedford, MA).
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2

Molecular Mechanisms of Oxidative Stress and Inflammation

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Idebenone (15475), mito-TEMPO (16621), and diphenyleneiodonium chloride (DPI; 81050) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). LPS (L2630) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and N-acetyl-L-cysteine (NAC; A0905) was purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). The following antibodies were used for Western blotting and immunohistochemical analyses: β-actin (sc-47778), SOD1 (sc-11407), SOD2 (sc-30080), Catalase (sc-50508), and α-tubulin (sc-8035) (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA); iNOS (13120s), COX-2 (12282s), TNF-α (3707s), IL-1β (12242s), IκBα (4812s), p-IκBα (2859s), IKKβ (2370s), p-IKKβ (2078s), p65 (8242s), p38 (8690s), p-p38 (4511s), JNK (9252s), p-JNK (4668s), ERK (4695s), p-ERK (4370p), AKT (4691s), and p-AKT (4060s) (Cell Signaling Technology Co., Danvers, MA, USA); and Lamin A (ab26300) (Abcam Ltd., Cambridge, UK). Permount was purchased from Fisher Scientific (Morris Plains, NJ, USA).
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