Df6009

MG132, cycloheximide (CHX), chloroquine (CQ), 4-Phenylbutyric acid (4-PBA), and Eeyarestatin I were obtained from MedChemExpress, and Dithiothreitol (DTT) was obtained from Yeasen. The transfection reagent jetPRIME was obtained from Polyplus. The antibodies were obtained commercially: anti-Flag mouse monoclonal antibody (F1804, Sigma), anti-ATF6 rabbit polyclonal antibody (DF6009, Affinity), anti-Hrd1 rabbit polyclonal antibody (A2605, Abclonal), anti-HA mouse monoclonal antibody (BD-PM2095, Biodragon), anti-GAPDH mouse monoclonal antibody (60004-1-Ig, Proteintech), HRP-conjugated goat anti-mouse IgG (BF03001, Biodragon), and fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (A0568, Beyotime). The mouse monoclonal antibody G3N against CDV NP was homemade in our laboratory (13 (link)).

Rabbit polyclonal antibodies against GRP78 (ab188878), CHOP (ab179823), and NURR1 (ab176184); the mouse monoclonal antibody against MAP2 (ab11268); and the rabbit monoclonal antibody targeting Ki-67 (ab16667) were purchased from Abcam (United States). Rabbit polyclonal antibodies against p-eIF2α (AF3087) and ATF6 (DF6009) were purchased from Affinity (China). The Alexa Fluor 488 donkey anti-mouse IgG (H + L) (1975519) and the Alexa Fluor 594 donkey anti-rabbit IgG (H + L) (1827674) secondary antibodies were purchased from Invitrogen (United States). The immunohistochemistry kit (SP9001) was purchased from the Zhongshan Goldenbridge Biotech, China, and the morphine hydrochloride for injection was produced in the First Pharmaceutical Factory of Shenyang, China.