Rna 6000 nano reagents and chips

Manufactured by Agilent Technologies

Sourced in Germany

The RNA 6000 Nano reagents and chips are designed for the analysis of total RNA samples. They provide a sensitive and automated method for the assessment of RNA quality and quantity using the Agilent Bioanalyzer system.

Automatically generated - may contain errors

Lab products found in correlation

Qubit rna high sensitivity assay, by Thermo Fisher Scientific (2 mentions) Rneasy mini column, by Qiagen (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Trizol method, by Thermo Fisher Scientific (1 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions)

Close spelling variants of this product

RNA 6000 Nano Chip RNA 6000 Nano Reagents 6000 Nano Chips RNA 6000 chips RNA Nano Chip RNA 6000 Nano RNA Nano reagents

2 protocols using rna 6000 nano reagents and chips

RNA Extraction from Malaria Parasites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen TRIzol-homogenized schizonts were thawed at room temperature and total RNA was extracted by the TRIzol method (Invitrogen Corp., Carlsbad, CA). RNA pellets were dissolved in 100 µL RNase-free water. The RNA was purified and DNA removed by DNase I digestion on RNeasy mini columns (Qiagen, Heidelberg, Germany), following which RNA was eluted in 50 µL RNase-free water, and quantified by Qubit High Sensitivity RNA Assay (Thermo Fisher Scientific). For samples containing at least 500 ng RNA, the RNA integrity was checked on an Agilent Bioanalyzer using RNA 6000 Nano reagents and chips (Agilent Genomics, Waldbronn, Germany).

Nyarko P.B., Tarr S.J., Aniweh Y., Stewart L.B., Conway D.J, & Awandare G.A. (2020). Investigating a Plasmodium falciparum erythrocyte invasion phenotype switch at the whole transcriptome level. Scientific Reports, 10, 245.

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RNA Extraction from Malaria Parasites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Erythrocytes containing the prepared schizonts were resuspended in 500 μl TRIzol® reagent (Thermo Fisher Scientific) and stored at − 80 °C until RNA extraction using RNeasy mini columns (Qiagen). RNA pellets were dissolved in 100 μl RNase-free H₂O, and a second RNA clean-up and on-column DNase treatment was carried out with RNA eluted in 30–50 μl RNase-free H₂O, and concentration quantified by Qubit High Sensitivity RNA Assay (Thermo Fisher Scientific). Samples containing at least 500 ng RNA were considered for RNA-seq processing after the RNA integrity was checked on an Agilent Bioanalyzer using RNA 6000 Nano reagents and chips (Agilent Genomics).

Tarr S.J., Díaz-Ingelmo O., Stewart L.B., Hocking S.E., Murray L., Duffy C.W., Otto T.D., Chappell L., Rayner J.C., Awandare G.A, & Conway D.J. (2018). Schizont transcriptome variation among clinical isolates and laboratory-adapted clones of the malaria parasite Plasmodium falciparum. BMC Genomics, 19, 894.

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