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Mouse apoe elisapro kit

Manufactured by Mabtech

The Mouse apoE ELISAPRO kit is a laboratory tool designed to detect and measure apolipoprotein E (apoE) levels in mouse samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to quantify the presence of apoE in a given sample.

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2 protocols using mouse apoe elisapro kit

1

Quantification of ApoE in Mouse Sera

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Mouse serum was collected from naïve WT C57/BL6 mice and also from both naïve and tumor-bearing C57/BL6 apoE-/- mice. ApoE levels in mouse sera were quantified using the mouse apoE ELISAPRO kit from Mabtech (Cincinnati, OH) as per the manufacturer’s directions.
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2

Intranasal Exposure to HDM and Poly(I:C) in Mice

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Female Balb/c mice (6 to 7 weeks of age) were purchased from Envigo (East Millstone, NJ) and experiments were performed using protocols approved by the Animal Care and Use Committee of the National Heart, Lung, and Blood Institute. Mice received intranasal administration of HDM, poly(I:C) (Sigma-Aldrich, St. Louis, MO), and/or APOE and bronchoalveolar lavage was performed. Urea was quantified in BALF and serum using the Urea Assay Kit (Sigma-Aldrich, St. Louis, MO) and the volume of ELF was determined as previously described30 (link). APOE was quantified using the Mouse apoE ELISAPRO Kit (Mabtech, Cincinnati, OH) and IL-1β was quantified using the Mouse IL-1β DuoSet ELISA (R&D Systems, Minneapolis, MN).
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