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Sx53g8

Manufactured by Abcam

The SX53G8 is a laboratory equipment product. It is designed to perform a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

2 protocols using sx53g8

1

Immunophenotyping of Cultured Cells

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Cultured cells and tissue sections prepared as previously described5 ,32 (link) stained for ER (SP1, ready to use), PR (1:50, PGR636), MUC1, (1:100, 115D8) and p27KIP1 (1:100, SX53G8, Abcam) by immunoperoxidase and for E-cadherin (1:10, HECD-1), vimentin (1:10, 3B4, Dako), MUC1 (1:10), K14 (1:50, LL002), K19 (1:50, human-specific, clone BA16) by fluorescence. Nuclei were counterstained with DAPI.
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2

Molecular Signaling in Tubular Epithelial Cells

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Human tubular epithelial cells HK‐2 were purchased from the America Tissue Culture Collection. The reverse transcription and SYBR Green qRT‐PCR kits were purchased from TOYOBO, Japan.
The following antibodies were used in this study: rabbit anti‐caspase3 antibody (1:1,000, 8G10, 9665; Cell Signaling Technology), rabbit anti‐cleaved‐PARP antibody (Asp214) (1:1,000, D6X6X, 94885, Cell Signaling Technology), rabbit antibody to beclin‐1 antibody (1:1,000, D40C5, 3495, Cell Signaling Technology), rabbit anti‐LC3B antibody (1:2,000, EPR18709, ab192890, Abcam), rabbit anti‐DCHS1 antibody (1:1,000, Ab203690; Abcam), rabbit anti‐cyclin D1 antibody (1:200, SP4, ab16663, Abcam), rabbit anti‐CDK4 antibody (1:2,000, EPR17525, ab199728, Abcam), mouse anti‐p27 antibody (1 μg/ml, SX53G8, ab193379, Abcam), and rabbit anti‐LC3B antibody (1 μg/ml, EPR18709, ab192890, Abcam).
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