Recombinant human TNF‐α (210‐TA) and CCL21 (366‐6C) were purchased from R&D Systems (Minneapolis, MN, USA). The following antibodies were used in Western blot analyses and immunocytochemistry: rabbit anti‐TNF‐α (ab6671), goat anti‐rabbit FITC‐conjugated IgG H&L (ab6717), and rabbit anti‐CCR7 (ab103404) from Abcam (Cambridge, MA, USA); rabbit anti‐p38 (8690), rabbit anti‐phospho‐p38 (Thr180/Tyr182; 4511), rabbit anti‐ERK (9102), rabbit anti‐phospho‐ERK (Thr202/Tyr204; 9101), and HRP‐conjugated anti‐rabbit IgG (7074) antibodies from Cell Signaling Technology (Danvers, MA, USA); and mouse anti‐GAPDH (NB300‐221) antibody from Novus Biologicals (Littleton, CO, USA). The ERK inhibitor U0126 (9903) and the p38 inhibitor SB203580 (5633) were purchased from Cell Signaling Technology.
Mouse anti gapdh nb300 221 antibody
Manufactured by Novus Biologicals
Sourced in United States
The Mouse anti-GAPDH (NB300-221) antibody is a primary antibody that specifically recognizes the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein. GAPDH is a widely expressed, highly conserved enzyme involved in glycolysis. This antibody can be used to detect and quantify GAPDH levels in various sample types through techniques such as Western blotting, immunohistochemistry, and ELISA.
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Lab products found in correlation
Hrp conjugated anti rabbit igg 7074, by Cell Signaling Technology (2 mentions)
Recombinant human tnfα 210 ta, by R&D Systems (1 mentions)
U0126 9903, by Cell Signaling Technology (1 mentions)
Sb203580 5633, by Cell Signaling Technology (1 mentions)
Ab9829, by Abcam (1 mentions)
Ab227036, by Abcam (1 mentions)
Ab175430, by Abcam (1 mentions)
Ab31630, by Abcam (1 mentions)
Ab64693, by Abcam (1 mentions)
Hrp conjugated anti mouse igg 1706516 antibody, by Bio-Rad (1 mentions)
Azd5363 s8019, by Selleck Chemicals (1 mentions)
2 protocols using mouse anti gapdh nb300 221 antibody
1
Signaling Pathways Activation in TNF-α and CCL21 Response
2
Characterization of CCL20-Mediated Signaling
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Recombinant human CCL20 (360-MP) was purchased from R&D Systems (Minneapolis, MN, USA). The following antibodies were used in western blot analyses, neutralizing assays, and immunohistochemistry: rabbit anti-CCL20 (ab9829), rabbit anti-CCR6 (ab227036), rabbit anti-CCR7 (ab103404), rabbit anti-CD206 (ab64693), mouse anti-CD68 (ab31630), mouse anti-Snail (ab117866), mouse anti-Twist (ab175430), and rabbit anti-Vimentin (ab92547), from Abcam (Cambridge, MA, USA); rabbit anti-Akt (9272S), rabbit anti-phospho-Akt (Ser473; 9271S), and HRP-conjugated anti-rabbit IgG (7074) antibodies from Cell Signaling Technology (Danvers, MA, USA); mouse anti-GAPDH (NB300-221) antibody from Novus Biologicals (Littleton, CO, USA); and HRP-conjugated anti-mouse IgG (1706516) antibody from Bio-Rad Laboratories (Hercules, CA, USA). The Akt inhibitor AZD5363 (S8019) was purchased from Selleck Chemicals (Houston, TX, USA).
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