Claspin

Manufactured by Fortis Life Sciences

Claspin is a lab equipment product from Fortis Life Sciences. It is a protein involved in the DNA damage response pathway. Claspin functions as a regulator of the ATR-Chk1 signaling cascade, which is important for checkpoint activation and DNA repair.

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Lab products found in correlation

Poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions) Hydroxyurea hu, by Merck Group (1 mentions) Ly294002, by Merck Group (1 mentions) Hct116, by National Collection of Authenticated Cell Cultures (1 mentions) Hepatocyte growth factor (hgf), by R&D Systems (1 mentions) Cdc45, by Santa Cruz Biotechnology (1 mentions) Chk1 ps317, by Cell Signaling Technology (1 mentions) Pold3, by Fortis Life Sciences (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions)

2 protocols using claspin

Colon Cancer Cell Line Characterization

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Colon cancer cells HT-29 and HCT-116 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Both cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium with 10% fetal bovine serum. HGF was purchased from R&D systems. LY294002 and hydroxyurea (HU) were purchased from Sigma-Aldrich. Antibodies to MET, phospho-MET (Tyr1234/1235), phospho-Chk1 (S345), AKT, phospho-AKT (Ser473), RAD51, poly (ADP-ribose) polymerase (PARP) were obtained from Cell Signaling Technology. Antibodies to Chk1, ATR, TopBP1, and Claspin were obtained from Bethyl. Antibodies to β-Tubulin and RAD51 (ab88572) were purchased from Abcam. Anti-Actin, secondary goat anti-rabbit, and goat anti-mouse antibodies were purchased from Santa Cruz Biotechnology.

Song N., Che X., Xu L., Qu J., Zheng H., Hou K., Qu X, & Liu Y. (2017). A novel function of hepatocyte growth factor in the activation of checkpoint kinase 1 phosphorylation in colon cancer cells. Molecular and Cellular Biochemistry, 436(1), 29-38.

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Replication Stress Response Profiling

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The following compounds were used: hydroxyurea (HU, 3mM), ATR inhibitor VE821 (ATRi, 3μM), aphidicolin (2.95μM), CDC7 inhibitor (CDC7i, 20μM) and roscovitine (20μM). HU and aphidicolin were used at 5mM and 14.75μM respectively for high dose replication stress. Antibodies used were CHK1 total (Santa Cruz), CHK1 pS317 (Cell Signaling), MCM2 (BD Biosciences), POLD3 (Bethyl), RPA2 (Abcam), PCNA (Santa Cruz), CDC45 (Santa Cruz), CLASPIN (Bethyl) and ORC2 (BD Biosciences).

Dungrawala H., Rose K.L., Bhat K.P., Mohni K.N., Glick G.G., Couch F.B, & Cortez D. (2015). The replication checkpoint prevents two types of fork collapse without regulating replisome stability. Molecular cell, 59(6), 998-1010.

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