Apc conjugated anti his antibody

Manufactured by BioLegend

The APC-conjugated anti-His antibody is a laboratory reagent designed for detection and analysis of proteins with a histidine (His) tag. It is a monoclonal antibody conjugated to the fluorescent dye allophycocyanin (APC), which allows for sensitive and specific identification of His-tagged proteins through flow cytometry or other fluorescence-based applications.

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Lab products found in correlation

Facscanto, by BD (1 mentions) Alexa fluor 647 protein labeling kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

APC-conjugated mouse anti-His antibody Anti-His-APC Anti-His antibody

2 protocols using apc conjugated anti his antibody

Quantifying uPAR Expression on Cells

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HEK-293T-uPAR-T2A-Luc or MKN-45-uPAR-T2A-Luc cells were seeded into a 10-cm dish. One day later, cells were trypsinized, washed twice with staining buffer (PBS plus 2% FBS), and split into three groups. One group (1 × 10⁶ cells in 100 μl of staining buffer) was treated with 1 μg of His-tagged pro-uPA (Sino Biological, 10815-H08H) plus 1 μg of the anti-uPAR mAb in the dark at 4°C for 30 min. The other two groups, the untreated group and the group treated with only 1 μg of His-tagged pro-uPA, were used as controls. After treatment, cells were washed thrice with the staining buffer and stained with APC-conjugated anti-His antibody (BioLegend, 362605). Pro–uPA-bound cells were detected using FCM (FACSCanto, BD Biosciences).

Qin L., Wang L., Zhang J., Zhou H., Yang Z., Wang Y., Cai W., Wen F., Jiang X., Zhang T., Ye H., Long B., Qin J., Shi W., Guan X., Yu Z., Yang J., Wang Q, & Jiao Z. (2022). Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer. Science Advances, 8(21), eabn3774.

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Recombinant Expression and Labeling of Bacterial Toxins

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The DT CRM-197 gene, a non-toxic mutant of DT, was amplified from a plasmid (a gift from Professor Eisuke Mekada) and cloned into the pCold II expression vector with 6× His-Tag at the N-terminal. The gene encoding the TT-binding domain was amplified from Clostridium tetani genomic DNA (a gift from Professor Tetsuya Iida) and cloned into the same expression system. These plasmids were then transformed into Escherichia coli BL21 and induced with IPTG at a final concentration of 0.5 mM for expression. The toxins were purified using AKTA Pure HisTrap Ni²⁺ affinity chromatography (Cytiva). The His-Tag is preserved and used to label the toxins by using APC-conjugated anti-His antibody (BioLegend). Purified PT (BIKEN) was stained using Alexa Fluor 647 Protein Labeling Kit (Invitrogen) according to manufacturer’s protocol.

Saputri D.S., Ismanto H.S., Nugraha D.K., Xu Z., Horiguchi Y., Sakakibara S, & Standley D.M. (2023). Deciphering the antigen specificities of antibodies by clustering their complementarity determining region sequences. mSystems, 8(6), e00722-23.

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