Zen 3.0 light microscopy software package

Stained tissue sections were observed on a ZEISS Axio Imager.Z2 equipped with a Zeiss alpha Plan-Apochromat objective 100×/1.46 Oil DIC M27, a Zeiss Objective Plan-Apochromat 150×/1.35 Glyc DIC Corr M27 and a ZEISS Axiocam 712 color digital microscope camera. Captured images were processed with the software program “Zen 3.0 Light Microscopy Software Package”, “ZEN Module Bundle Intellesis & Analysis for Light Microscopy”, “ZEN Module Z Stack Hardware” (Carl Zeiss Vision, Jena, Germany) and submitted with the final revision of the manuscript at 300 DPI.

The stained tissue sections were observed on a ZEISS Axio Imager.Z2 that was equipped with a Zeiss alpha Plan-Apochromat objective 100×/1.46 Oil DIC M27, a Zeiss Objective Plan-Apochromat 150×/1.35 Glyc DIC Corr M27, and a ZEISS Axiocam 712 color digital microscope camera. The captured images were processed with the software programs, “Zen 3.0 Light Microscopy Software Package”, “ZEN Module Bundle Intellesis & Analysis for Light Microscopy”, “ZEN Module Z Stack Hardware” (Carl Zeiss Vision, Aalen, Germany), and submitted with the final revision of the manuscript at 300 DPI. Photomicrographs were obtained in some cases with a confocal microscope, Nikon D-Eclipse C1 Si based on Nikon “Eclipse 90i”. The number of mast cells in the different areas of the ovary was determined per mm² of tissue, using open-source software for the digital pathology image analysis, QuPath [90 (link)] (Bankhead P., et al., 2017) (Table 1).

Stained tissue sections were observed on a ZEISS Axio Imager.A2 equipped with a Zeiss alpha Plan-Apochromat objective 100x/1.46 Oil DIC M27, a Zeiss Objective Plan-Apochromat 150x/1.35 Glyc DIC Corr M27 and a ZEISS Axiocam 712 color digital microscope camera. Captured images were processed with the software program «Zen 3.0 Light Microscopy Software Package», «ZEN Module Bundle Intellesis & Analysis for Light Microscopy», «ZEN Module Z Stack Hardware» (Carl Zeiss Vision, Germany) and submitted with the final revision of the manuscript at 300 DPI. The number of collagen fibers with different morphometric characteristics in the pericellular region of the mast cells was determined using open-source software for digital pathology image analysis QuPath [42 (link)], with further calculation of the relative content (Table 1).