Jms d 300 spectrometer

All chemical were purchased from the Merck, Sigma-Aldrich, and used without further purification. Solvent were dried and distilled prior to use. Merck pre-coated silica gel plates with a fluorescent indicator were used for analytical TLC. Flash column chromatography was performed using silica gel (Merck). Ethyl acetate – hexane was used as a eluting solvent for TLC and column chromatography. Melting points were recorded in open capillary tubes and were uncorrected. The FT-IR spectra (KBr) were recorded on a Shimadzu 8201pc (4000–400 cm⁻¹) spectrometer. The ¹H NMR (Proton Nuclear Magnetic Resonance Spectra) were obtained on a Bruker DRX-300 MHz and those of ¹³C NMR (Carbon Nuclear Magnetic Resonance Spectra) were recorded on a Bruker DRX-75 MHz advance spectrometer. Chemical shifts of ¹H and ¹³C Nuclear Magnetic Resonance spectra were expressed in ppm in downfield from tetramethylsilane. Mass spectrum (EI) was recorded on a Jeol JMS D-300 spectrometer operating at 70 eV. The elemental analysis (C, H, N, and S) were recorded using an Elemental analyzer model (Varian EL III) and agreed with the calculated values.

All chemicals were purchased from Merck and Sigma–Aldrich, and were used without further purification. Pre-coated silica gel plates impregnated with a fluorescent indicator were used for analytical TLC. An ethyl acetate/hexane solvent mixture was used as the eluent for both TLC and column chromatography. The FT–IR spectra were recorded using KBr pellets on a Shimadzu 8201pc spectrometer operating between 4000 and 400 cm⁻¹. The ¹H and ¹³C NMR spectra were obtained on Bruker DRX-300 MHz and Bruker DRX-75 MHz Avance spectrometers, respectively, and the resulting chemical shifts were expressed in ppm using tetramethylsilane as the internal standard. JEOL JMS D-300 spectrometer was used to analysis the mass spectra (EI) all compounds. Elemental analysis (C, H, N, and S) was conducted using a Varian EL III elemental analyzer.