Methanol

Chitosan produced from Aspergillus niger (Deacetylation degree (DD) = 92%, determined by FT-IR method; Mw = 890,000 g/mol; and 100–300 cps), fluorescein, L-aspartic, and L-glutamic acid were purchased from PolAura, Dywity, Poland. Propylene glycol, isopropanol, methanol (HPLC grade), (CH₃COO)₂Zn∙2H₂O, CaCl₂·2H₂O, MgSO₄, KCl, KOH, ethanol (96%, 99.9%), KHPO₄, Na₂HPO₄, NaHCO₃, NaCl, HCl, NaOH, mouse L929 (commercial cell line from mouse C3H/An connective tissue for research purpose only, The European Collection of Authenticated Cell Cultures (ECACC)), Dulbecco’s Modified Eagle Medium (DMEM) with glucose content cell culture medium, antibiotics (streptomycin/penicillin), fetal bovine serum (FBS), phosphate buffer solution (PBS), trypsin with EDTA, and Triton-X-100 were purchased from Sigma Aldrich, Poznań, Poland. For the study, pure cannabidiol of HPLC confirmed purity was used, purchased from Dobre Konopie, Mysłowice, Poland.

Monoclonal antibodies (developed in mice) against Streptococcus agalactiae were obtained from ThermoFisher Scientific (Warsaw, Poland). The ND powder (monocrystalline Diamond Podwer (0–0.03 micron were obtained from Pureon (Lengwil, Switzerland). Potassium hexacyanoferrate (III), methanol, sodium nitrite and hydrochloric acid were purchased from Chempur (Piekary Śląskie, Poland). Phosphate-buffered saline (PBS), 4-aminobenzoic acid (4-ABA), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), Tris-buffered saline (TBS) and sodium azide (NaN₃) were obtained from Sigma–Aldrich (Poznan, Poland). Alumina slurries of 0.3 m were purchased from Buehler (Lake Bluff, IL, USA). Sulphuric acid, potassium hydroxide, hydrogen peroxide, ethanol and methanol were supplied by Pol-Aura (Warsaw, Poland). The potato starch (PS) was purchased at a local supermarket from Grula^® Trzemeszno, Poland).