Sh igf2bp2

All animal experiments were conducted based on protocols approved by the ethical committee of Animal Experiment Center of Hangzhou Medical College (SCXK (zhe) 2019–0,002). For the subcutaneously implanted tumor assay, 1 × 106 H1299/R cells co-transfected with sh-LINC01001, sh-IGF2BP2 or pcDNA-LINC01001 or mock vector which were purchased from Shanghai GenePharma Co., Ltd. They were resuspended in 100 μl PBS and subcutaneously injected into the left flanks of 4 week-old female BALB/c nude mice (7 mice per group). Afterwards, the nude mice were obtained and raised in the Model Animal Research Center of Nanjing University. Tumor growth was monitored once a week. The nude mice were sacrificed after 15 days, and the tumor specimens were weighed, fixed and then stained by H&E staining for histological analyses. The immunohistochemical (IHC) staining was performed to evaluate the levels of Ki67 in tumor tissues, according to the published protocol (Ding et al., 2003 (link)).

short hairpin RNA (Sh)-METTL14, sh-YTHDF1, sh-YTHDF2, sh-YTHDC1, sh-IGF2BP1, sh-IGF2BP2, sh-IGF2BP3, sh-NC, empty vector, and SMAD1 overexpression vector, IGF2BP1 overexpression vector, and IGF2BP2 overexpression vector were obtained from Genepharma. BMSCs were seeded into 6-well plates and transfected with sh-METTL14 and sh-NC using Lipofectamine 3000 (Invitrogen, Carlsbad) for 48 h.