Determination of nuclear position and cross wall orientation of cells was carried out using an AxioImager.Z1 Apotome microscope (Zeiss, Jena, Germany) with a ×20 objective and digital image acquisition controlled by AxioVision Software 4.8 (Zeiss). For examination of the mitotic spindle, confocal z-stacks were recorded at day 1 after subcultivation in the AtTUB6–GFP tobacco BY-2 strain using a Zeiss Observer.Z1 microscope with a Yokogawa CSUx1 detection system comprising a ×63 LCI-Neofluar Imm Corr DIC objective (NA 1.3) and the 488nm emission line of the Ar–Kr laser, as well as a spinning-disc device (YOKOGAWA CSU-X1 5000). Analysis of KCH binding on MTs under parthenolide treatment was performed using the same settings. Images were recorded at day 3 after subcultivation under treatment with 10 μM parthenolide. Untreated OsKCH cells at day 3 served as a control.
Observer z1 microscope
Manufactured by Yokogawa
The Observer Z1 is a microscope designed for high-performance imaging and analysis. It features a high-resolution optical system and advanced camera capabilities for accurate visualization and data collection.
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Lab products found in correlation
Axio imager z1 apotome microscope, by Zeiss (1 mentions)
Axiovision software 4, by Zeiss (1 mentions)
Csu x1 5000, by Yokogawa (1 mentions)
Csu x1 a1 confocal scanner unit, by Yokogawa (1 mentions)
Celldiscoverer 7, by Zeiss (1 mentions)
Dmi6000b inverted microscope, by Leica (1 mentions)
Csu x1 spinning disc unit, by Yokogawa (1 mentions)
Efficient navigation zen , by Zeiss (1 mentions)
C11440, by Hamamatsu Photonics (1 mentions)
Orca flash 4.0 camera, by Hamamatsu Photonics (1 mentions)
3 protocols using observer z1 microscope
1
Imaging Mitotic Spindle and KCH Binding
2
Visualizing V. dahliae Colonization in A. thaliana
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A. thaliana (Col-0, N1902; Nottingham Arabidopsis Stock Centre) seedlings were inoculated by root-dipping with a conidia suspension of V. dahliae JR2 and NLP3 deletion strain overexpressing GFP (1 × 107 spores/ml) based on the method described by Bui et al. (2019) (link). 3-week-old seedlings were used for infection. The roots were incubated in spore solutions with 100 000 spores/ml for 35 min. The plates were further incubated in the plant chamber at long day conditions (22−25°C) and colonization on the roots was monitored at indicated time points. The root was incubated in a staining solution (0.0025% (v/v) propidium iodide, 0.005% (v/v) silwet) for 5 min in the dark. Images of infected roots were taken with a Zeiss Observer Z1 microscope equipped with CSU-X1 A1 confocal scanner unit (Yokogawa), QuandtEM:512SC (Photometrics) digital camera and Slidebook 5.0 software package (Intelligent Imaging Innovations).
3
Live Imaging of Embryonic Development
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For live imaging, embryos are placed in 5 cm glass-bottom dishes (MatTek) under a CellDiscoverer 7 (Zeiss) equipped with a 20x/0.95 objective and an ORCA-Flash 4.0 camera (C11440, Hamamatsu) or a 506 axiovert (Zeiss) camera. Using the experiment designer tool of ZEN (Zeiss), we set up nested time-lapses in which all embryos are imaged every 5 h for 10 min with an image taken every 5 s at 2 focal planes positioned 10 μm apart. Embryos are kept in a humidified atmosphere supplied with 5% CO2 at 37°C. mTmG embryos are imaged at the 2-and 16cell stage using an inverted Zeiss Observer Z1 microscope with a CSU-X1 spinning disc unit (Yokogawa). Excitation is achieved using a 561 nm laser through a 63x/1.2 C Apo Korr water immersion objective. Emission is collected through 595/50 band pass filters onto an ORCA-Flash 4.0 camera (C11440, Hamamatsu). The microscope is equipped with an incubation chamber to keep the sample at 37°C and supply the atmosphere with 5% CO2. Surface tension measurements are performed on a Leica DMI6000 B inverted microscope equipped with a 40x/0.8 DRY HC PL APO Ph2 ( 11506383) )objective and Retina R3 camera and 0,7x lens in front of the camera. The microscope is equipped with an incubation chamber to keep the sample at 37°C and supply the atmosphere with 5% CO2.
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