Kinetex c18 100a

Manufactured by Phenomenex

Sourced in United States

The Kinetex C18 100A is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a porous core-shell particle design that provides high-speed and high-efficiency separations for a variety of small molecules. The column has a C18 stationary phase and a 100Å pore size.

Automatically generated - may contain errors

Lab products found in correlation

Tripletof 5600, by AB Sciex (1 mentions) Speed vacuum, by Thermo Fisher Scientific (1 mentions) Qtrap 6500, by AB Sciex (1 mentions) Multiquant, by AB Sciex (1 mentions) Prominence system, by Shimadzu (1 mentions) 1290 infinity lc system, by Agilent Technologies (1 mentions) 6490 triple quadrupole lc ms system, by Agilent Technologies (1 mentions) Masshunter software, by Agilent Technologies (1 mentions) Agilent 1260 infinity hplc system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Kinetex 5-μm C18 100A column Kinetex XB-C18 100A Kinetex 5μm EVO C18 100A Kinetex 5u EVO C18 100A column Kinetex 5u EVO C18 100A 250 × 4.6 mm Kinetex 2,6u XB-C18 100A Kinetex EVO C18 100A Kinetex 2.6 µm C18 100A column Kinetex 2.6 μm XB-C18 100A reverse phase column Kinetex 5u Evo C18 100A

6 protocols using kinetex c18 100a

High-Resolution Mass Spectrometry Analysis of Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The UHPLC-HRMS/MS analyses of sample extracts (70% methanol) were
performed in a hybrid quadrupole-TOF LC/MS/MS mass spectrometer system
(Sciex Triple ToF 5600, Singapore), which was connected to a Kinetex
C18 100A (30 mm × 2.1 mm, 1.7 μm) Phenomenex column at
a flow rate of 0.4 mL/min. The mobile phase comprises solvent A 0.1%
acetic acid in water and solvent B 0.1% acetic acid in acetonitrile
and methanol in an 8:2 ratio.^{23 (link)} The column
temperature was maintained at 35 °C, and the mass measurements
were taken with a scan range of 200–2000 m/z and an IRDx resolution of 15 000 in the
ESI negative mode. GS1-45, GS2-60, and Curtain GAS (CUR)-40 were used
as gases. The duospray ion source was set with an ion spray voltage
floating (ISVF) of 4500 at 400 °C. The cycle time was 700 ms,
the accumulation time was 250.0 ms, and the accumulation time was
30 min. The information-dependent acquisition (IDA) procedure employed
the most intense ion with spread energy at 20% and normal collision
energy at 45%. The mass measurements were recorded with a scan range
of 200–2000 m/z in the ESI
negative mode. The data were analyzed using Peak View 2.1 software
(AB SCIEX Triple TOF 5600, Singapore) and equipped with MasterViewTM
(Version 1.0, AB SCIEX). The XIC manager tool in Master View was used
to detect quasi-molecular weights, mass errors, and isotope patterns
of both nontargeted and targeted compounds.

Chaya H.C., Kumar S.S., Jayarama S, & Mahadevappa P. (2022). Comprehensive Nutritional Analysis, Antioxidant Activities, and Bioactive Compound Characterization from Seven Selected Cereals and Pulses by UHPLC-HRMS/MS. ACS Omega, 7(35), 31377-31387.

+ Open protocol

+ Expand

HPLC Analysis of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HPLC analyses of samples were performed on an Agilent 1200 HPLC instrument connected with a diode array detector (DAD). The columns were Phenomenex Kinetex C18 100A (Phenomenex, Torrance, CA, USA) reversed-phase columns (100 × 4.60 mm, 5 µm). The injection volume was 20 µL. The gradient mobile phase was: 30% MeCN/H₂O for 2 min, 30% MeCN/H₂O to 95% in 12 min, 95% MeCN/H₂O for 15 min, 95% MeCN/H₂O to 30% MeCN/H₂O in 0.2 min, and finally 30% MeCN/H₂O for 2.8 min with a flow rate of 0.6 mL/min. The MeCN was added with 0.1% HCOOH.

Wang Y., Zhou L., Chen M., Liu Y., Yang Y., Lu T., Ban F., Hu X., Qian Z., Hong P, & Zhang Y. (2023). Mining Xanthine Oxidase Inhibitors from an Edible Seaweed Pterocladiella capillacea by Using In Vitro Bioassays, Affinity Ultrafiltration LC-MS/MS, Metabolomics Tools, and In Silico Prediction. Marine Drugs, 21(10), 502.

+ Open protocol

+ Expand

Quantification of Thyroid Hormones by LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

T₄ and T₃ were simultaneously measured by liquid chromatography (dual LC Shimadzu Prominence system, Shimadzu, Columbia, MD) followed by tandem mass spectrometry (Ferrara et al., 2013 (link)). (Q-Trap 6500, Sciex) with a TurboVion source. After addition of the internal standard ¹³C₆ - T₃ and ¹³C₆- T₄ (Iso Sciences) to 100 μL sample of Transport Buffer collected from the top or bottom chamber, the iodothyronines were extracted with 1:4 (v/v) and with 100 μL of EtOH:NH₄OH (98:2). The combined supernatants were evaporated using a speed vacuum (Thermo Scientific). The residue was then reconstituted in 100 μL of 0.1% Formic acid in water and 40 μL of reconstituted extract was injected into Kinetex C18–100A (2.6 μ, 30 × 3 mm, Phenomenex, CA) column, protected by a Phenomenex C18-RP guard cartridge in 40°C column oven. lodothyronines were chromatographed with 0.1% formic acid in deionized water (aqueous mobile phase A) and 0.1% formic acid in methanol (organic mobile phase B). The gradient was 5 to 90% B in 2 min with flow rate of 0.4 ml/min. The positive ion multiple reaction monitoring (MRM) mode was used for detection. The MRM transition monitored was: m/z 777.5 > 731.5 for T₄; m/z 651.5 > 605.5 for T₃; m/z 651.5 > 605.5 for ¹³C₆ - T₃, and m/z 783.5 > 737.3 for ¹³C₆- T₄. All the MRM data was processed with Multiquant (Sciex).

Vatine G.D., Al-Ahmad A., Barriga B.K., Svendsen S., Salim A., Garcia L., Garcia V.J., Ho R., Yucer N., Qian T., Lim R.G., Wu J., Thompson L.M., Spivia W.R., Chen Z., Van Eyk J., Palecek S.P., Refetoff S., Shusta E.V, & Svendsen C.N. (2017). Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier. Cell stem cell, 20(6), 831-843.e5.

+ Open protocol

+ Expand

Quantification of Bioactive Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

To perform a quantification analysis, 1 mg of KYQG extract was ultrasonically extracted with 9 ml of 50% methanol in volumetric flask (10 ml) for 30 min and then diluted to volume. The mixture was diluted to appropriate concentration filtered through a 0.22 µm filter for quantification analysis. The stock solutions of arginine, liquiritin, chlorogenic acid, harpagoside, luteolin-7-O-glucoside, and glycyrrhizic acid were prepared in methanol and then mixed and diluted to serial concentrations in 50% methanol. Linearity of the method was examined by using the serial mixed standards and the calibration curves were calculated by the least squares linear regression method. The separation of the sample was conducted on a Phenomenex Kinetex C18 100A (2.10mm × 100 mm, 2.6 μm, Torrance, USA). The mobile phase consisted of acetonitrile containing 0.1% formic acid (mobile phase A) and water containing 0.1% formic acid (mobile phase B), and the gradient elution program was as follows: 0–30 min, 5 to 62% A; 30–33 min, 62% to 100% A. The ﬂow rate was 0.3 mL/min, and the sample injection volume was 5 μL. MS conditions were set as described above (Part 2.1).

Chen P., Yao H., Su W., Zheng Y., Fan W., Zhang L., Chen T., Wu S., Zhang W., He Y., Yan Z., Wang Y, & Li P. (2020). Pharmacodynamic and Metabolomics Studies on the Effect of Kouyanqing Granule in the Treatment of Phenol-Induced Oral Ulcer Worsened by Sleep Deprivation. Frontiers in Pharmacology, 11, 824.

+ Open protocol

+ Expand

LC-MS/MS Analysis of Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

The evaporated samples were dissolved in 40 μl of mobile phase prior to LC-MS/MS analysis, using a 1290 Infinity LC system and a 6490 Triple Quadrupole LC/MS system equipped with Jet Stream and Dual Ion Funnel systems (Agilent Technologies, http://www.home.agilent.com). A 20 μl portion of each sample was injected onto a reversed-phase column (Kinetex C18 100A, length 50 mm, diameter 2.1 mm, particle size 1.7 μm; Phenomenex, http://www.phenomenex.com), and the analytes were eluted by a 3 min linear gradient of 5:95 to 35:65 A:B, where A and B are 0.1% acetic acid in methanol and 0.1% acetic acid in water, respectively. The column was then washed with 100% methanol (1.0 min), and re-equilibrated to initial conditions (1.0 min). Throughout the procedure, the flow rate was 0.5 ml min⁻¹, and the column temperature 40 °C. The effluent was introduced into the MS system with the optimized settings listed in Supplementary Table S2 at JXB online. Analytes were quantified using diagnostic multiple reaction monitoring (MRM) transitions of precursor and appropriate product ions using optimal collision energies and 50 ms dwell time (Table S2). Chromatograms were analysed using MassHunter software (version B.05.02; Agilent Technologies), and the compounds were also quantified by standard isotope dilution analysis (Rittenberg and Foster, 1940 ).

Pěnčík A., Casanova-Sáez R., Pilařová V., Žukauskaitė A., Pinto R., Micol J.L., Ljung K, & Novák O. (2018). Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis. Journal of Experimental Botany, 69(10), 2569-2579.

+ Open protocol

+ Expand

HPLC Analysis of Derivatized Amines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analyses were carried out on an Agilent 1260 Infinity HPLC system (Agilent Technologies, Santa Clara, CA, USA) consisted of the quaternary pump VL (G1311C), a standard
autosampler (G1329B), a thermostatted column compartment (G1316A) and a fluorescence detector (G1321B). A Kinetex® C18 100A (150 mm length × 4.6 mm i.d., 5.0 µm particle diameter) analytical column (Phenomenex, Torrance, CA, USA) was used for the separation of the derivatized amines. A Velp Scientifica RX3 Vortex shaker (Milan, Italy), a water bath equipped with a Digiterm 200 immersion thermostat (JP Selecta S.A., Barcelona, Spain), and a Sigma 2-16K refrigerated centrifuge (Osterode, Germany) were used.

Herrero A., Sanllorente S., Reguera C., Ortiz M.C, & Sarabia L.A. (2016). A new multiresponse optimization approach in combination with a D-Optimal experimental design for the determination of biogenic amines in fish by HPLC-FLD. Analytica chimica acta, 945.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!