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Ctss activity kit

Manufactured by Abcam
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Sourced in Germany

The CTSS activity kit is a laboratory tool designed to measure the enzymatic activity of CTSS, a cysteine protease. The kit provides the necessary reagents and protocols to quantify CTSS activity in biological samples, allowing researchers to study its role in various physiological and pathological processes.

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Lab products found in correlation

Lactoferrin hlf2 human elisa kit, by Abcam (1 mentions) Proparacaine 0.5, by Alcon (1 mentions)

2 protocols using ctss activity kit

1

Quantifying Tear Biomarkers for Clinical Insights

Cited 1 time
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CTSS activity was determined with the CTSS activity kit (BioVision) according to the manufacturer’s instructions. Activity values were normalised to total protein concentration as described previously5 (link). sIgA, LF, and Cys C levels in tears eluted from Schirmer’s strips were quantified using the sIgA ImmuChrom ELISA Kit (Eagle Biosciences, Amhurst NH), lactoferrin (HLF2) human ELISA Kit (Abcam), and Cys C quantikine human Cys C ELISA kit (R&D Systems), respectively. Final tear dilutions were 1:5,000 for sIgA, 1:60,000 for LF, and 1:50 for Cys C.
Edman M.C., Janga S.R., Meng Z., Bechtold M., Chen A.F., Kim C., Naman L., Sarma A., Teekappanavar N., Kim A.Y., Madrigal S., Singh S., Ortiz E., Christianakis S., Arkfeld D.G., Mack W.J., Heur M., Stohl W, & Hamm-Alvarez S.F. (2018). Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sjögren’s Syndrome patients. Scientific Reports, 8, 11044.
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2

Tear Protein Extraction for CTSS Assay

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Topical anesthetic (Proparacaine 0.5%, Alcon, Fort Worth, TX) without fluorescein was applied to the eyes and tears were collected on Schirmer’s strips for 5 min. The strips were incubated in CTSS reaction buffer, obtained with the CTSS activity kit (BioVision, Milpitas, CA), and centrifuged at 6700 RCF for 15 sec in an Eppendorf (Hamburg, Germany) minispin centrifuge to elute proteins.
Edman M.C., Janga S.R., Meng Z., Bechtold M., Chen A.F., Kim C., Naman L., Sarma A., Teekappanavar N., Kim A.Y., Madrigal S., Singh S., Ortiz E., Christianakis S., Arkfeld D.G., Mack W.J., Heur M., Stohl W, & Hamm-Alvarez S.F. (2018). Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sjögren’s Syndrome patients. Scientific Reports, 8, 11044.
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