Mouse anti β actin monoclonal antibody

For western blotting (WB), the proteins were resolved on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels followed by standard WB. Rabbit anti-6×His tag antibody (Abcam, Cambridge, MA, USA) (1:2,000) as the primary antibody. Goat anti-rabbit IgG H&L (Abcam, Cambridge, MA, USA) (1:4,000) was used as the secondary antibody and a SuperSignal West Femto Trial Kit (Thermo Fisher Scientific, Waltham, MA, USA) was used for color development. β-Actin protein was used as the control, using mouse anti-β-actin monoclonal antibody (Sino Biological, Beijing, China) (1:2,000) and goat anti-mouse IgG H&L (Abcam, Cambridge, MA, USA) (1:4,000).

Immunoblotting analysis of the Ats-1 protein was performed through SDS-PAGE (CWBIO, Beijing, China) using mouse anti-6 × His-tag antibody (Abcam, Cambridge, MA, USA) (1:1000) as the primary antibody. Goat Anti-mouse IgG H&L (Abcam, Cambridge, MA, USA) (1:3000) was implemented as the secondary antibody, and a SuperSignal West Femto Trial Kit (ThermoFisher, Waltham, MA, USA) was utilized for color development. Actin was utilized as a control. Mouse Anti-β-actin Monoclonal Antibody (Sino Biological, Beijing, China) (1:1000) was used as the primary antibody and Goat Anti-mouse IgG H&L (Abcam, Cambridge, MA, USA) (1:3000) was used as the secondary antibody in our research. And the blots were cut prior to hybridisation with antibodies during blotting.

The antibodies and drugs used in this study included rabbit monoclonal anti-Oct-1 antibody (Abcam, ab178869), rabbit monoclonal anti-HCF-1 antibody (Abcam, ab289975), mouse monoclonal anti-VP16 antibody (Santa Cruz, sc7545), mouse monoclonal anti-β-actin antibody (Sino Biological, 1000166), rabbit monoclonal anti-GAPDH antibody (Abways Technology, AB0037), mouse monoclonal anti-Histone-H3 antibody (Sino Biological, 100005-MM01), rabbit polyclonal antibody anti-TSG101 (Proteintech, 28283-I-AP), mouse monoclonal anti-HSV-1-gD antibody (Santa Cruz, sc21719), mouse monoclonal anti-ICP0 antibody (Santa Cruz, 13,118), Alexa Fluor 594-labeled goat anti-rabbit IgG (H + L; Invitrogen, 2165334), Alexa Fluor plus 488-labeled goat anti-mouse IgG (H + L; Invitrogen, A32723), goat anti-mouse IgG-HRP (Invitrogen, 31430), goat anti-rabbit IgG (H + L)-HRP (Invitrogen, 32460), and protease inhibitor cocktail (Thermo Scientific, EO0492).