Cell proliferation of the RWPE-1 cells was monitored with an xCELLigence RTCA MP Instrument (Roche Diagnostics GmbH, Berlin, Germany) for 48 h. Background impedance was measured in 100 μL of culture medium, and the final volume was adjusted to 200 μL (5 × 103 cell/well). The impedance was recorded every 15 min. The cell index was normalized to the impedance at the time point of the TP or TP + VA administration.
Xcelligence rtca mp instrument
Manufactured by Roche
Sourced in Germany
The XCELLigence RTCA MP Instrument is a label-free, real-time cell analysis system used for monitoring cell proliferation, cytotoxicity, and cell-based assays. The instrument measures and records changes in electrical impedance, which reflects the changes in cell number, morphology, and attachment on specialized microelectronic plates. It provides real-time quantitative data on these cellular parameters without the need for any labels or dyes.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using xcelligence rtca mp instrument
1
Monitoring RWPE-1 Cell Proliferation
2
Monitoring Cell Growth Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell growth behavior was continuously monitored for 72 h using the xCELLigence RTCA MP Instrument (Roche Diagnostics GmbH, Germany). Background impedance was measured in 100 μl cell culture medium per well. The final volume was adjusted to 200 μl cell culture medium, including 5 × 103 cells/well. After plating, impedance was recorded in 15 min intervals. All experiments were performed in triplicates. Cell Index (CI) values were normalized to the time point of 100 and 300 μM of BSN administration (referred to as normalized CI).
3
Real-Time Monitoring of Cell Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell growth behavior was continuously monitored for 48 hours using the xCELLigence RTCA MP Instrument (Roche Diagnostics GmbH, Mannheim, Germany). Background impedance was measured in 100 µL cell culture medium per well. The final volume was adjusted to 200 µL cell culture medium, including 5 × 103 cells/well. After plating, impedance was recorded in 15-minute intervals. All experiments were performed in triplicates. Cell Index (CI) values were normalized to the time point of 200 µg/mL of SEYG administration (referred to as normalized CI).
4
Cell Migration Assay Using xCELLigence
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the migration assay the cells were transfected as described above. Cells were cultured in serum free medium for 24h. After the starving 5 × l04 HEC1B cells in 100 μ1 were seeded in a CIM 16 well E-plate. The continuous migration of the cells was documented and analysed in a time-resolved manner with the xCELLigence RTCA MP instrument and the RTCA Software 1.2 (Roche diagnostics, Mannheim, Germany) to measure the CI values. The migration was measured over a time period of 48h. During the measurement the lower chambers contained full growth medium as chemoattractant and the upper chambers medium without FCS.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!