Triethylammonium salt texas red dhpe

L-α-Lysophosphatidylcholine,N,N-dicyclohexylcarbodiimide (DCCI), 4,4′-dimethylamin-pyridine (DMAP), cholesterol, and dichloromethane (CH₂Cl₂) were purchased from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). 5-Hexynoic acid was purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). Silica gel (60/200) was purchased from Kanto Chemical Co. (Tokyo, Japan). Sulforhodamine101-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red-DHPE) and RhodamineB-tagged dextran (zwitterionic, molecular weight = 10000) were purchased from ThermoFisher Scientific, Inc. (Waltham, MA, USA). Tris-EDTA Buffer 10x powder (pH 7.4) was purchased from Takara Bio Inc. (Shiga, Japan). Lambda phage DNA (48502-base pair) methylated from Escherichia coli host strain W3110 was purchased from Sigma-Aldrich (St. Louis, MO, USA). SYBR® Green I was purchased from LONZA (Basel, Switzerland). Water was distilled and deionized before use with a MilliQ system from Millipore (Billerica, MA, USA). All reagents were used as received. L-α-Lysophosphatidylcholine was an almost equal amount mixture of 3-(palmitoyloxy)-2-hydroxypropyl (2-(trimethylammonio)ethyl) phosphate, and 3-(stearoyloxy)-2-hydroxypropyl (2-(trimethylammonio)ethyl) phosphate, which was determined by electrospray ionization mass spectrometry using a model LCMS-2020 device (Shimadzu, Kyoto, Japan).

To prepare the solutions, 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) as solutions in chloroform and 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DOPG) were purchased from Avanti Polar Lipids. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red-DHPE), and calcein (>93%) were purchased from Thermo Fisher Scientific (Monza, Italy). chloroform, 1-octanol, and glycerol were acquired from Honeywell (Milan, Italy), and sucrose ultrapure was obtained from VWR-Life Science. Milli-Q water obtained using a purification system (Fulltech Instruments, Rome, Italy) was used for the preparation of all aqueous solutions.
The lipid mixture (DOPC:DOPG in a 1:1 ratio with 0.1 mol% Texas Red-DHPE) was dissolved in chloroform in a glass vial. chloroform was evaporated under a gentle stream of nitrogen, and the lipids were further dried by desiccating for at least 1 h. After adding octanol in order to achieve a final lipid concentration of 15 mg/mL, the lipid–oil solution (LO) was sonicated for 30 min and then filtered using 0.45 µm syringe filters. The internal aqueous solution (Wi) was 600 mM sucrose with 0.05 mM calcein, and the external aqueous solution (We) was 600 mM glucose.