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Sodium hydrogen phosphate na2hpo4

Manufactured by Merck Group
Sourced in Germany

Sodium hydrogen phosphate (Na2HPO4) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. The primary function of sodium hydrogen phosphate is to serve as a buffering agent, maintaining a specific pH level in various chemical reactions and applications.

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5 protocols using sodium hydrogen phosphate na2hpo4

1

Functionalization of Biomolecules with NHS

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N-Hydroxysuccinimide (NHS) was obtained from Fluka Chemie AG, Buchs, Switzerland. Acetic acid, ammonium chloride (NH4Cl), ammonium sulfate ((NH4)2SO4), bovine serum albumin, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), ethanol, glutaraldehyde, hydrogen chloride, nitric acid, paraformaldehyde, sodium chloride (NaCl), sodium hydrogen phosphate (Na2HPO4) and sodium sulfate (Na2SO4) were from Merck, Darmstadt, Germany. Glycine was from Scharlau Chemicals, Barcelona, Spain. Barium perchlorate (Ba(ClO4)2), barium sulfate (BaSO4), calcium chloride (CaCl2), calcium sulfate (CaSO4), magnesium chloride (MgCl2), magnesium perchlorate (Mg(ClO4)2), 2-(N-morpholino)ethane sulfonic acid (MES) and trinitrobenzene sulfonic acid (TNBS) were from Sigma Aldrich, St. Louis, MO, USA. Barium chloride (BaCl2) and magnesium sulfate (MgSO4) were from VWR International, Radnor, PA, USA.
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2

Analytical Cannabis and Opioid Detection

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Water purity was 18.2 mΩ/cm (Millipore, Molsheim, France). Methanol was supplied by Biosolve (Dieuze, France). Formic acid and orthophosphoric acid were ordered to Carlo Erba (Val de Reuil, France). Acetonitrile and sodium dihydrogen phosphate (NaH2PO4) were supplied by Fisher Scientific (Illkirch, France). Sodium hydrogen phosphate (Na2HPO4) was ordered from Merck (Darmstadt, Germany). β-glucuronidase was purchased by MP Biomedicals (Illkirch, France). LGC standards (Molsheim, France) supplied vials for 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol-D3 (THC-COOH-D3), Δ9-tetrahydrocannabinol-D3 (THC-D3), methamphetamine, cocaine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymetamphetamine (MDMA), MDMA-D5, morphine-D3, buprenorphine, methadone, 6-monoacetylmorphine (6-MAM), 6-MAM-D3. Vials of 11-OH-THC, THC-COOH, THC, methampethamine-D5, amphetamine, amphetamine-D5, cocaine, cocaine-D3, MDA-D5, benzoylecgonine, benzoylecgonine-D3, morphine, buprenorphine-D4 and methadone-D3 were supplied by Euromedex (Souffelweyersheim, France).
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3

Synthesis and Characterization of PBI Polymer

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PBI (purity >99.95%, molecular formula: (C20H12N4)n, MW 51,000,) was purchased from Danish Power Systems. Lithium chloride (LiCl), N,N-dimethylacetamide (DMAc) 99.8%, phosphoric acid (extra pure, 85% solution in water), phytic acid solution (50% (w/w) in H2O), sodium hydrogen phosphate (Na2HPO4), and sodium tungstate dihydrate (Na2WO4·2H2O) were purchased from Sigma-Aldrich (Aldrich, Madrid, Spain). The phosphotungstic acid solution was prepared using Na2WO4·2H2O and Na2HPO4 as precursors, by mixing 1.10 g of Na2HPO4 and 5.95 g of Na2WO4·2H2O in 30 mL of deionized water at 50 °C.
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4

Analytical Method for Sulfonamide Antibiotics

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Ammonium molybdate tetrahydrate (H24Mo7N6O24·4H2O) (99%), thioacetamide (TAA) (98%), sulfathiazole (STZ), sulfadimidine (SDD), sulfadiazine (SDZ), sulfamethoxazole (SMZ), sulfacetamide (ST), sulfachloropyridazine (SCD), N4-phthalylsulfathiazole (PST), succinylsulfathiazole (SST), sodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), methanol for HPLC (99.9%) and acetonitrile for HPLC (99.9%) were purchased from Sigma-Aldrich (St Louis, USA). Acetone, sodium hydroxide (NaOH) and hydrochloric acid (HCl) were purchased from Beijing Chemical Works (Beijing, China). Polyethylene glycol (PEG4000) was purchased from Merck (Germany). Ultrapure water (18.2 MΩ cm) was prepared using a Milli-Q Gradient ultrapure water system (Millipore, Milford, MA, USA).
The eight SAs were dissolved in a 0.1 mol l−1 NaOH solution to prepare standard stock solutions of 2.0 mg ml−1 for each SA, and these standard solutions were stored at 4°C and protected from light. The working solutions to be analysed were diluted with ultrapure water. The calibration standards of SAs were prepared with five levels of concentration in the range of 0.3 to 30 µg ml−1.
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5

Synthesis and Characterization of Metallic Nanoparticles

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Palladium(ii) chloride (PdCl2) was purchased from Alfa Aesar. Ruthenium(iii) chloride hydrate (RuCl3·xH2O), polyvinylpyrrolidone (PVP), potassium tetrachloroplatinate(ii) (K2PtCl4), sodium dihydrogen phosphate (NaH2PO4·H2O), and sodium hydrogen phosphate (Na2HPO4) were obtained from Sigma-Aldrich. Potassium hydroxide (KOH), hydrogen peroxide (H2O2), and hydrochloric acid (HCl, 35–37%) were acquired from Duksan. Hydrazine (N2H4, 80%) was supplied by TCI. The carbon support (Vulcan XC-72) and commercial Pt/C (with a 20 wt% metal loading on Vulcan XC-72) were produced by Premetek. The compositions and morphologies of all synthesized samples were analyzed by high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) and transmission electron microscopy (TEM, Hitachi, HF-3300). The structural properties and phases of all samples were determined by powder X-ray diffraction (XRD) using a Diatome MPD for bulk powder. X-ray photoelectron spectroscopy (XPS) was carried out using a ULVAC-PHI Quantera SXM. The Brunauer–Emmett–Teller (BET) surface area and pore size distributions of catalysts were analyzed using a BELSORP-mini II (Mictrotrac-BEL, Japan). All solutions were prepared using purified water (Milli-Q, 18 MΩ cm).
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