The effect of the treatment on the ATT parameter dynamic measured by point Shear-Wave Elastometry (pSWE) was considered the primary outcome. Examinations were performed using a Fujifilm Arietta 850 (Tokyo, Japan) ultrasound machine by two certified sonographers (OO and MM), blinded to randomization. During expiration, ten sequential measurements were obtained via an intercostal approach in the upper portion of the right lobe. The measurement sites were at least 2 cm below the liver capsule and at a depth of less than 6 cm below the skin. Using the reliability index, the percentage of the net amount of effective shearwave velocity (VsN) helped us confirm whether pSWE was appropriate [8] . Examinations were performed on the first day and at the end of the study. The secondary outcomes were the effect of synbiotics on the lipid profile, HbA1c, inflammatory markers, estimated glomerular filtration rate (eGFR), and the microbiome dynamic. Stool and serum sampling was performed twice, on the day when the first therapeutic package was issued and within 48 hours after the last drug dose.
Arietta 850
Manufactured by Fujifilm
Sourced in Japan
The ARIETTA 850 is a diagnostic ultrasound system designed for general diagnostic applications. It provides high-quality imaging capabilities to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
Gf uct260, by Olympus (4 mentions)
Thinprep cytolyt solution, by Hologic (1 mentions)
Expecttm, by Boston Scientific (1 mentions)
Acquiretm, by Boston Scientific (1 mentions)
Gf ue260 al5, by Olympus (1 mentions)
Prosound ssd α 10, by Aloka (1 mentions)
Aplio mx, by Canon (1 mentions)
Prosound f75, by Fujifilm (1 mentions)
7 protocols using arietta 850
1
Synbiotic Effects on Liver Elasticity
2
EUS-FNA for Pancreatic Lesion Diagnosis
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Patients underwent EUS-FNA using a GF-UCT260 linear echoendoscope (Olympus Medical, Tokyo, Japan) connected to an ultrasound scanning system (ARIETTA 850; Fujifilm, Tokyo, Japan). The pancreatic lesion was punctured using a 22/25G aspiration needle (ExpectTM or AcquireTM; Boston Scientific, Natick, MA, USA; or EZ Shot3TM; Olympus Medical, Tokyo, Japan) under real-time ultrasound guidance. The stylet was withdrawn and aspirated (10 cc negative pressure) using the attached syringe and the aspiration needle was moved back and forth 20 times within the lesion before being withdrawn from the echoendoscope. Finally, the aspirated material was pushed out into a preservative liquid (ThinPrep CytoLyt Solution; Hologic, Marlborough, MA, USA) by reinsertion of the stylet.
The aspirated material was separated for histological evaluation, cytological evaluation and Kras mutation analysis. The solid materials were fixed in 10% formalin, embedded in paraffin and sliced thinly for additional immunostaining as required. The liquid material was treated using the ThinPrep® method according to the manufacturer’s recommendations and then evaluated immediately by Papanicolaou staining. The residual LBC specimens were stored at 4 °C until DNA extraction.
The aspirated material was separated for histological evaluation, cytological evaluation and Kras mutation analysis. The solid materials were fixed in 10% formalin, embedded in paraffin and sliced thinly for additional immunostaining as required. The liquid material was treated using the ThinPrep® method according to the manufacturer’s recommendations and then evaluated immediately by Papanicolaou staining. The residual LBC specimens were stored at 4 °C until DNA extraction.
3
Endoscopic Ultrasound Evaluation of Solid Pancreatic Lesions
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All endoscopic examinations were performed or supervised by experienced endosonographers. EUS was performed using ARIETTA 850 (FUJIFILM Healthcare, Tokyo, Japan) and GF-UCT260 (Olympus Medical Systems, Tokyo, Japan). Propofol, midazolam, and/or diazepam were administered to induce sedation. The doses were adjusted according to the body weights and ages of the patients. The SPLs, including the surrounding pancreatic parenchyma, were scanned using B-mode to acquire high-quality ultrasound images of the scan region. eFocusing was implemented during the acquisition. eFLOW was set to approximately 5.0 cm/s in the velocity range. Subsequently, DFI was used to evaluate the vascular structure within and surrounding the SPLs. The optimal color gain was adjusted based on the size and depth of the SPLs. A region of interest (ROI), including the SPLs and adjacent pancreatic parenchyma, was defined for each SPL. All images were stored in a digital format on an ARIETTA 850 hard disk by each endosonographer. In addition, static vessel images of the largest plane of each SPL were acquired for blinded reading.
4
Contrast-Enhanced Harmonic EUS Assessment
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Electronic radial-type or convex-type echoendoscopes (GF-UE260-AL5, GF-UCT260; Olympus, Tokyo, Japan) with an ultrasound processor (ALOKA ProSound SSD α-10; Aloka Co. Ltd., Tokyo, Japan; ARIETTA 850; FUJIFILM Healthcare, Tokyo, Japan) were used for the EUS procedures which were performed by several endosonographers who had at least 10 years of experience in EUS. The latter employed the extended pure harmonic detection method with the mechanical index set at 0.25. After reconstitution with 2 mL of sterile water for injection, 0.7 mL of the contrast agent (Sonazoid®; GE Healthcare Pharm, Tokyo, Japan) was administered through a peripheral vein for CH-EUS. Vascularity and vascular pattern were assessed by two endosonographers (who had at least 10 years EUS experience) after recording the EUS images. When independently gained assessments differed between the two reviewers, reevaluation was performed between the reviewers until an agreement was reached.
5
Contrast-Enhanced Endoscopic Ultrasound Imaging
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Convex-type endoscopes (GF-UCT260; Olympus, Tokyo, Japan) with ultrasound observation systems (ARIETTA 850; FUJIFILM Healthcare, Tokyo, Japan) were used. The patient was placed in the left lateral position under diazepam-induced sedation for EUS, and heart rate, saturation, and blood pressure were monitored. The color gain, dynamic range, and transmission frequency were set to 65, 83, and 5.0 MHz, respectively, for DFI-EUS, and 52, 83, and 5.0 MHz for e-FLOW EUS. The color gain settings were optimized before starting the study to minimize the noise in lesions in either mode, and these values were then fixed across all patients. EUS images were recorded for the subsequent evaluation of vascularity. Assessment of vessels was performed on the recorded EUS images by three endosonographers who had at least 10 years of EUS experience, and who were blinded to all other imaging and patient information. Vessels were assessed as absent/present or regular/irregular vessels. The terms regular vessel images refers to smooth and linear vessels in the tumor, while irregular vessels are defined as tortuous vessels with a caliber change (Figure 2 ).
6
Bilateral Limb Vein Ultrasonography Protocol
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Ultrasonography were obtained using the Aplio MX (CANON Medical Systems Corporation), the Prosound F75 (FUJIFILM Healthcare Corporation), and the ARIETTA 850 (FUJIFILM Healthcare Corporation) with the linear probe.
Bilateral lower limb vein ultrasonography was performed preoperatively and postoperatively 1 week after THA. A medical technologist (MT) performed the ultrasonography, another MT confirmed the results. Moreover, the report is finally approved by the cardiologist.
The femoral vein, superficial femoral vein, deep femoral vein, popliteal vein, peroneal vein, anterior tibial vein, posterior tibial vein, SV, large saphenous vein, and small saphenous vein were all examined. In the supine position, we checked the thigh, while in the sitting position, we checked the lower leg beyond the popliteal vein. Thrombus screening was performed in B mode using the compression and Doppler methods. When an intravenous thrombus image and intravascular noncompression findings were observed, a thrombus-positive diagnosis was made when an intravenous thrombus image and intravascular noncompression findings were observed15 (link).
Bilateral lower limb vein ultrasonography was performed preoperatively and postoperatively 1 week after THA. A medical technologist (MT) performed the ultrasonography, another MT confirmed the results. Moreover, the report is finally approved by the cardiologist.
The femoral vein, superficial femoral vein, deep femoral vein, popliteal vein, peroneal vein, anterior tibial vein, posterior tibial vein, SV, large saphenous vein, and small saphenous vein were all examined. In the supine position, we checked the thigh, while in the sitting position, we checked the lower leg beyond the popliteal vein. Thrombus screening was performed in B mode using the compression and Doppler methods. When an intravenous thrombus image and intravascular noncompression findings were observed, a thrombus-positive diagnosis was made when an intravenous thrombus image and intravascular noncompression findings were observed15 (link).
7
Detailed Axillary Ultrasound Protocols
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One of the two attending breast radiologists (J.M.C. and S.M.H., with 15 and 9 years of experience in breast imaging, respectively) performed axillary US using a 13–5-MHz linear transducer (Arietta 850; Fujifilm Health Care). Details on standard axillary US, including B-mode and color Doppler images, are described in Appendix E1 (online).
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