A total of 482 NSCLC specimens with ALK gene status evaluated by FISH were collected and stored in the Biobank of Korea University Guro Hospital between 2012 and 2018. The glass slides were reviewed for histological diagnosis and immunohistochemical features, including ALK (5A4; Novocastra), TTF-1 (8G7G3/1; Dako; Agilent Technologies, Inc.), and napsin A (polyclonal; Cell Marque). The formalin-fixed paraffin-embedded (FFPE) tissue blocks of 10 patients, stored for <3 years to minimize the degradation of DNA and RNA, were selected for NGS analysis, and consisted of five ALK FISH-positive and five ALK FISH-negative adenocarcinomas (38 (link),39 (link)). The clinical information included age, sex, smoking history, cancer stage according to the 8th Edition of the American Joint Committee on Cancer staging manual, genetic mutation status and survival. All the glass slides, paraffin blocks and clinical information were provided by the Human Biobank of Korea University Guro Hospital, which collects patients' samples and medical information at the time of biopsies or surgical excisions of their tumors, with the written informed consent for future research use. The present study was conducted with the approval of the Institutional Review Board of Korea University Guro Hospital (approval no. 2018GR0357).
Ttf 1 8g7g3 1
Manufactured by Agilent Technologies
Sourced in Denmark
The TTF-1 (8G7G3/1) is a lab equipment product offered by Agilent Technologies. It serves as a core component in various laboratory applications. The detailed technical specifications and intended use of this product are not available in an unbiased and factual format without further interpretation.
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Lab products found in correlation
3 protocols using ttf 1 8g7g3 1
1
NSCLC Biobank Tissue Analysis
2
Thyroid Carcinoma Cases with CRCC-like Appearance
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We reviewed 12,064 primary thyroid carcinoma cases resected in Kuma Hospital from 2005 to 2015, and selected two cases (cases 1 and 2) with CRCC-like appearance. CRCC-like appearance was defined as a prominent cell border, perinuclear clear cytoplasm, a wrinkled nuclear membrane, and binucleation. One case (case 3) from Fukushima Medical University School of Medicine with similar histological findings was also included in this study. Immunohistochemical study was performed using following antibodies, thyroglobulin (polyclonal, 2 dilution, Dako, Glostrup, Denmark), TTF1 (8G7G3/1, 100 dilution, Dako Cytomation, Carpinteria, CA, USA), EMA (E29, 5 dilution, Dako, Carpinteria, CA, USA), PAX8 (polyclonal, 200 dilution, Protein Tech, Chicago, IL, USA), cytokeratin 7 (OV-TL 12/30, 50 dilution, DAKO, Carpinteria, CA, USA), cytokeratin
3
Immunohistochemistry Staining Protocol for Tissue Analysis
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Immunohistochemical staining was performed on 4-μmthick sections, cut from formalin-fixed, paraffin-embedded tissue, using a Ventana BenchMark XT automated stainer (Ventana Medical Systems, Tucson, AZ), according to the manufacturer'sp r o t o c o l .
The following antibodies were used: S-100 protein (polyclonal; RTU; Ventana), STAT5 (E289, 1:500, AbCam), mammaglobin (clone 304-1A5;R T U ;D a k o C y t o m a t i o n ) , NTRK (A7H6R, 1:25; Cell Signaling), GATA3 (clone L50-823; 1:200; BioCareMedical), CK7 (clone OV-TL 12/30; 1:200; DakoCytomation), TTF-1 (8G7G3/1, 1:100, Dako), p63 (clone 4A4; RTU; Ventana), and CD117 (polyclonal, 1:800, Dako). The panel varied between individual cases depending on the origin.
The following antibodies were used: S-100 protein (polyclonal; RTU; Ventana), STAT5 (E289, 1:500, AbCam), mammaglobin (clone 304-1A5;R T U ;D a k o C y t o m a t i o n ) , NTRK (A7H6R, 1:25; Cell Signaling), GATA3 (clone L50-823; 1:200; BioCareMedical), CK7 (clone OV-TL 12/30; 1:200; DakoCytomation), TTF-1 (8G7G3/1, 1:100, Dako), p63 (clone 4A4; RTU; Ventana), and CD117 (polyclonal, 1:800, Dako). The panel varied between individual cases depending on the origin.
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