Mbs c001

Manufactured by ACROBiosystems

MBS‐C001 is a laboratory equipment item from ACROBiosystems. It is a core device used for biological and biochemical applications. The MBS‐C001 performs essential functions required in research and development settings.

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Lab products found in correlation

Thp 1, by American Type Culture Collection (1 mentions) Easysep cd8 t cell isolation kit, by STEMCELL (1 mentions)

2 protocols using mbs c001

Culturing Epithelial, Leukemia, and T Cells

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Human nasopharyngeal epithelial cells NP69 were procured from BeNa Culture Collection. NPC cell lines HNE3, C666‐1, and HK1 were procured from Chuan Qiu Biotechnology. These cells were cultured in Dulbecco's modified Eagle's medium along with 10% fetal bovine serum (FBS). Monocytic leukemia cells (THP‐1) and CD8⁺ cytotoxic T cells were procured from American Type Culture Collection. After cell recovery in Roswell Park Memorial Institute‐1640, the THP‐1 cells were induced with 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) to differentiate into macrophages, and the CD8⁺ T cells were activated and expanded by CD3/CD28 magnetic beads (MBS‐C001, Acro Biosystems) stimulation. All cells were maintained in a 37°C incubator with 5% CO₂.

Wang Q., Yu Q, & Liu Y. (2023). E2F3 renders an immunosuppressive tumor microenvironment in nasopharyngeal carcinoma: Involvements of the transcription activation of PRC1 and BIRC5. Immunity, Inflammation and Disease, 11(8), e987.

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Exosome-mediated CD8+ T cell activation

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Isolation of CD8⁺ T cells from human peripheral blood mononuclear cells (PBMCs) was performed using the Easy‐Sep™ CD8⁺ T cell Isolation Kit (Stemcell Technologies). Exosomes derived from TNBC cells were added into a 12‐well plate and co‐cultured with CD3/28 bead (MBS‐C001; AcroBiosystems) preactivated CD8⁺ T cells. Further analysis was performed after incubation for 24 h.

Li W., Han G., Li F., Bu P., Hao Y., Huang L, & Bai X. (2023). Cancer cell‐derived exosomal miR‐20a‐5p inhibits CD8 + T‐cell function and confers anti‐programmed cell death 1 therapy resistance in triple‐negative breast cancer. Cancer Science, 115(2), 347-356.

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