6-OHDA hydrobromide, tubastatin A, and sodium hydrogen sulfide were obtained from Sigma Aldrich. RNA isolation kits, cDNA synthesis, and SYBR Green/ROX master mix were from Qiagen. Primer sequences for PCR were obtained from Eurofins MWG (Operon). Protein carbonyl, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) assays kits were obtained from Cayman Chemicals, USA. The HDAC activity assay kit was obtained from Biomol, PA, USA. The HAT activity assay kit was acquired from Active Motif, Carlsbad, CA, USA. The Fluoro-Jade C assay kit was obtained from Millipore Sigma, Burlington, MA, USA. GFAP monoclonal antibody and the corresponding anti-mouse secondary antibody were obtained from Santa Cruz Biotechnology, USA. ELISA kits for IL-1β (ER1094), IL-2 (ER0039), IL-17 (ER0035), IL-8 (ER1623), IL-6 (ER0042), TH (ER0534), GFAP (ER0229), α-synuclein (ER0921), and TNF-α (ER1393) were obtained from Fine Biotech, China. All other chemical reagents used were analytical grade.
Gfap monoclonal antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The GFAP monoclonal antibody is a laboratory reagent used for the detection and analysis of glial fibrillary acidic protein (GFAP) in various applications. GFAP is a type III intermediate filament protein that is expressed in astrocytes and other glial cells. The GFAP monoclonal antibody can be utilized in techniques such as immunohistochemistry, Western blotting, and flow cytometry to identify and quantify GFAP in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rna isolation kit, by Qiagen (1 mentions)
Sodium hydrogen sulfide, by Merck Group (1 mentions)
Protein carbonyl, by Cayman Chemical (1 mentions)
Sybr green rox master mix, by Qiagen (1 mentions)
Tubastatin a, by Merck Group (1 mentions)
6 ohda hydrobromide, by Merck Group (1 mentions)
Hat activity assay kit, by Active Motif (1 mentions)
Apolyvinylidene difluoride membrane, by Merck Group (1 mentions)
Universal hood 2 gel imager, by Bio-Rad (1 mentions)
2 protocols using gfap monoclonal antibody
1
Oxidative Stress Biomarkers in Neurodegenerative Models
2
GFAP and GAPDH Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell samples
were harvested and lysed with a protein extraction solution (Beijing
Kangwei Century, China) consisting of protease and phosphatase inhibitors.
According to the size of protein molecular weight, the appropriate
concentration of sodium dodecyl sulfate-polyacrylamide gel electrophoresis
gel was prepared for protein separation and then transferred to a
poly(vinylidene difluoride) membrane (Millipore, USA), which was closed
with 5% skimmed milk for 4 h; the membrane was incubated with GFAP
monoclonal antibody (1:5000, Santa Cruz Biotechnology, USA) and control
anti-GAPDH (1:3000, CWBIO, China) overnight at 4 °C; the bound
antibodies were detected using HRP-labeled sheep antirabbit IgG (1:3000,
CWBIO, China) and HRP-labeled sheep antimouse IgG (1:3000, CWBIO,
China), and the bound antibodies were detected using ultrasensitive
electrochemical luminescence chemiluminescence solution (1:3000, CWBIO,
China) on a Universal Hood II gel imager (Bio-Rad, USA) for protein
banding image acquisition.
were harvested and lysed with a protein extraction solution (Beijing
Kangwei Century, China) consisting of protease and phosphatase inhibitors.
According to the size of protein molecular weight, the appropriate
concentration of sodium dodecyl sulfate-polyacrylamide gel electrophoresis
gel was prepared for protein separation and then transferred to a
poly(vinylidene difluoride) membrane (Millipore, USA), which was closed
with 5% skimmed milk for 4 h; the membrane was incubated with GFAP
monoclonal antibody (1:5000, Santa Cruz Biotechnology, USA) and control
anti-GAPDH (1:3000, CWBIO, China) overnight at 4 °C; the bound
antibodies were detected using HRP-labeled sheep antirabbit IgG (1:3000,
CWBIO, China) and HRP-labeled sheep antimouse IgG (1:3000, CWBIO,
China), and the bound antibodies were detected using ultrasensitive
electrochemical luminescence chemiluminescence solution (1:3000, CWBIO,
China) on a Universal Hood II gel imager (Bio-Rad, USA) for protein
banding image acquisition.
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