Mouse pups were anesthetized and transcardially perfused with 4% paraformaldehyde in PBS. The brain tissues were embedded in paraffin and sliced coronally in 5 μm. Tissue sections were dewaxed, quenched with 3% hydrogen peroxide for 10 min, and incubated with 5% bovine serum albumin (BSA) for 30 min. The sections were stained overnight at 4 °C with either anti-ST2 (1:200, ab25877, Abcam), anti-IL-33 (1:500, AF3626, R&D), anti-GFAP (1:500, 104805-T08, Sino Biological Inc.), anti-Olig2 (1:500, EPR2673, Abcam), anti-Iba1(1:50, 20A12.1, Sigma-Aldrich), anti-Neun (1:50, A60, Sigma-Aldrich), or anti-Ki67 (1:500, ab15580, Abcam) followed by PE- or FITC-conjugated secondary antibodies (eBioscience). TUNEL staining was performed with the in situ cell death detection kit (Roche). The slides were counterstained with nuclear dye DAPI and observed on an Olympus BX51 fluorescent microscope.
Pe or fitc conjugated secondary antibodies
Manufactured by Thermo Fisher Scientific
PE- or FITC-conjugated secondary antibodies are laboratory reagents used to detect and visualize target proteins or molecules in various experimental techniques, such as flow cytometry, immunofluorescence, and Western blotting. These antibodies are conjugated with either the fluorescent dyes Phycoerythrin (PE) or Fluorescein Isothiocyanate (FITC), which allow for the specific labeling and detection of the target of interest.
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2 protocols using pe or fitc conjugated secondary antibodies
1
Immunohistochemical Analysis of Mouse Brain
2
Immunohistochemical Analysis of Mouse Brain
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Mouse pups were anesthetized and transcardially perfused with 4% paraformaldehyde in PBS. The brain tissues were embedded in paraffin, sliced coronally in 5-μm. Tissue sections were dewaxed, quenched with 3% hydrogen peroxide for 10 min, and incubated with 5 % bovine serum albumin (BSA) for 30 min. The sections were stained overnight at 4°C with either anti-ST2 (1:200, ab25877, Abcam), anti-IL-33 (1:500, AF3626, R&D), anti-GFAP (1:500, 104805-T08, Sino Biological Inc.), anti-Olig2 (1:500, EPR2673, Abcam), anti-Iba1(1:50, 20A12.1, Sigma-Aldrich), anti-Neun (1:50, A60, Sigma-Aldrich), or anti-Ki67 (1:500, ab15580, Abcam) followed by PE-or FITC-conjugated secondary antibodies (eBioscience). TUNEL staining was performed with the in situ cell death detection kit (Roche). The slides were counterstained with nuclear dye DAPI and observed on an Olympus BX51 fluorescent microscope.
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