Cell light edu apollo 488 or 567 in vitro imaging kit

Cell proliferation capacity was determined by MTT assay. Cells were seeded in 96-well plates at a density of 3,000 cells/well. After incubation, each well was added using MTT (5 mg/mL) (Sigma-Aldrich, MO, USA), and incubated for 4 h. At the end of incubation, supernatants were removed, and dimethyl sulfoxide (Sigma-Aldrich, MO, USA) was added to each well. The absorbance value (OD) of each well was measured at 490 nm. The calculated rates were then used for curve fitting. All assays were independently performed three times. For EdU assay, proliferating NSCLC cells were examined using the Cell-Light EdU Apollo 488 or 567 In vitro Imaging Kit (RiboBio).

The Cell-Light EDU Apollo 488 or 567 in vitro Imaging kit (Guangzhou Ribobio Co., Ltd.) was used to measure glioma cells proliferation, according to the manufacturer’s introduction. Briefly, following incubation with 10 mM EdU for 2 h at 37°C with 5% CO₂, the U87 and LN229 cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100 (0.2%), and stained with Apollo fluorescent dyes and costained with 5 μg/mL DAPI. Finally, EdU-positive cells were counted under a fluorescence microscope in five random fields. All experiments were independently performed at least three times.