Ethylnitrosourea

Manufactured by Merck Group

Sourced in Japan

Ethylnitrosourea is a chemical compound used as a laboratory reagent. It is a colorless crystalline solid. The primary function of ethylnitrosourea is as a DNA alkylating agent, which is commonly used in various scientific research applications.

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Lab products found in correlation

Male c57bl 6j mice, by Merck Group (2 mentions) Ethyl methanesulphonate, by Merck Group (1 mentions) Cyclophosphamide, by Merck Group (1 mentions)

3 protocols using ethylnitrosourea

Ethylnitrosourea-Induced Mutagenesis in Mice

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Male C57BL/6J mice (CLEA Japan) were treated with ethylnitrosourea (85 mg/kg, Sigma-Aldrich) by intraperitoneal injection twice at weekly intervals at the age of 8 weeks. At the age of 25-30 weeks, the sperm of the mice was used for in vitro fertilization with eggs of C57BL/6N mice to obtain F1 offspring. Mice were provided food and water ad libitum, and were maintained on a 12-hour light/dark cycle and housed under controlled temperature and humidity conditions. All procedures were approved by the Institutional Animal Care and Use Committee of the University of Tsukuba and the RIKEN BioResource Center, University of Texas Southwestern Medical Center at Dallas.

Funato H., Miyoshi C., Fujiyama T., Kanda T., Sato M., Wang Z., Ma J., Nakane S., Tomita J., Ikkyu A., Kakizaki M., Hotta N., Kanno S., Komiya H., Asano F., Honda T., Kim S.J., Harano K., Muramoto H., Yonezawa T., Mizuno S., Miyazaki S., Connor L., Kumar V., Miura I., Suzuki T., Watanabe A., Abe M., Sugiyama F., Takahashi S., Sakimura K., Hayashi Y., Liu Q., Kume K., Wakana S., Takahashi J.S, & Yanagisawa M. (2016). Forward genetic analysis of sleep in randomly mutagenized mice. Nature, 539(7629), 378-383.

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ENU-Induced Germ Cell Mutagenesis

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Male C57BL/6J mice (CLEA Japan) were treated with ethylnitrosourea (85 mg/kg BW, Sigma-Aldrich) via intraperitoneal injections twice at weekly intervals at 8 weeks old17 (link). At the age of 25–30 weeks, the sperm of the mice were collected and used for the in vitro fertilization of eggs from C57BL/6N mice to obtain F1 offspring. Mice were provided food and water ad libitum, maintained on a 12-hour light/dark cycle and housed under controlled temperature and humidity conditions. All procedures were carried out in accordance with the Guidelines for Animal Experiments of the University of Tsukuba and the RIKEN Tsukuba Institute and were approved by the Institutional Animal Care and Use Committee of the University of Tsukuba and the RIKEN Tsukuba Institute.

Hossain M.S., Asano F., Fujiyama T., Miyoshi C., Sato M., Ikkyu A., Kanno S., Hotta N., Kakizaki M., Honda T., Kim S.J., Komiya H., Miura I., Suzuki T., Kobayashi K., Kaneda H., Kumar V., Takahashi J.S., Wakana S., Funato H, & Yanagisawa M. (2016). Identification of mutations through dominant screening for obesity using C57BL/6 substrains. Scientific Reports, 6, 32453.

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NDMA Mutagenicity Assay Protocol

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N-Nitrosodimethylamine (NDMA, CAS no. 62-75-9) purity 95% (Batch Number, 2020-0369507) was purchased from Enamine Ltd (Kyiv, Ukraine) and was stored at approximately 4°C protected from light. Dose formulations were prepared in 0.5% (w/v) aqueous methylcellulose (400 cps at 2%) and were stable and homogenous for 8 days when protected from light and stored at 15–25°C or for 16 days at 2–8°C. Fresh formulations were prepared weekly for the main Muta™Mouse study. Ethyl nitrosourea, cyclophosphamide, and ethyl methane sulphonate were purchased from Sigma (UK).

Lynch A.M., Howe J., Hildebrand D., Harvey J.S., Burman M., Harte D.S., Chen L., Kmett C., Shi W., McHugh C.F., Patel K.K., Junnotula V., Kenny J., Haworth R, & Wills J.W. (2024). N-Nitrosodimethylamine investigations in Muta™Mouse define point-of-departure values and demonstrate less-than-additive somatic mutant frequency accumulations. Mutagenesis, 39(2), 96-118.

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