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2 protocols using ab126181

1

Comprehensive Mitochondrial Protein Analysis

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For Western blot analysis, the following antibodies were used in a 1:1000 dilution: anti-β (ab14730, Abcam), anti-γ (PA5-29975, ThermoFisher), anti-b (ab117991, Abcam), anti-OSCP (ab110276, Abcam), anti-f (ab200715, Abcam), anti-g (ab126181, Abcam), anti-e (ab122241, Abcam), anti-c (ab181243, Abcam), anti-a (ab192423, Abcam), anti-citrate synthase (ab96600, Abcam), anti-vinculin (V4505, Sigma), anti-prohibitin (MS-261-P1, NeoMarkers), anti-GAPDH (2118, Cell Signaling), anti-CyPD (ab110324, Abcam), anti-ANT2 (14671, Cell Signaling), anti-ANT3 (PA5-35113, ThermoFischer), anti-OXPHOS (ab110411, Abcam), anti-HKII (sc130358, Santa Cruz), anti-UQCRC1 (sc65238, Santa Cruz), anti-Grim19 (sc271013, Santa Cruz), and anti-SDHA (sc166947, Santa Cruz). Statistical comparison of data was assessed with the two-sided Student’s t-test.
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2

Antibody Validation for Cell Biology

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Recombinant TGF-β (TGF-β3) was purchased from R&D systems (Minneapolis, MN, USA). The following antibodies were used: mouse anti-α-tubulin (DM1A; Sigma-Aldrich, St Louis, MO, USA), mouse anti-TTF-1 (8G7G3/1; Novus Biologicals, Littleton, CO, USA), rabbit anti-RBM47 (Sigma-Aldrich, HPA006347), anti-Nrf2 (EP1808Y; Abcam, Cambridge, UK), anti-KEAP1 (10503-2-AP; Proteintech, Chicago, IL, USA), anti-CUL3 (Abcam, EPR3196Y), anti-HDAC1 (2E10; Millipore, Billerica, MA, USA), anti-ATP5L (Abcam, ab126181) and anti-NDUFS6 (Abcam, ab156099).
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