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Tax1bp1

Manufactured by Novus Biologicals
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TAX1BP1 is a protein that functions as an adapter protein, facilitating interactions between other proteins. It plays a role in various cellular processes, including signal transduction and protein trafficking. The core function of TAX1BP1 is to mediate protein-protein interactions, though its specific applications may vary depending on the research context.

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Lab products found in correlation

Hsp60, by Santa Cruz Biotechnology (2 mentions) Caspase 3, by Novus Biologicals (2 mentions) Ndp52, by BD (2 mentions) Phospho aktser473, by Novus Biologicals (2 mentions) Phospho mtorser2448, by Novus Biologicals (2 mentions) Phospho p70 s6 kinasethr389, by Novus Biologicals (2 mentions) Phospho ulkser757, by Novus Biologicals (2 mentions) Optineurin, by Thermo Fisher Scientific (2 mentions) Caspase 9, by Novus Biologicals (2 mentions) Beclin 1, by Cell Signaling Technology (2 mentions) Pink1, by Proteintech (2 mentions) Image lab analysis software, by Bio-Rad (2 mentions) Gapdh, by Novus Biologicals (2 mentions) Chemidoc molecular imager, by Bio-Rad (2 mentions) Criterion tgx gel, by Bio-Rad (2 mentions) Trans blot turbo system, by Bio-Rad (2 mentions)

2 protocols using tax1bp1

1

Immunoblotting of Autophagic Proteins

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Approximately 15 μg of protein was loaded in precast 4–20% Criterion TGX gels (Bio-Rad Laboratories, Hercules, CA), transferred to a PVDF membrane with the Trans-BlotⓇ Turbo™ system (Bio-Rad Laboratories), blocked, incubated with primary antibodies, and incubated with the secondary antibody. Images were captured via the ChemiDoc Molecular Imager and bands were quantified with ImageLab analysis software (both from Bio-Rad). Expression levels were determined by chemiluminescent immunodetection and normalized to appropriate loading controls. Immunoblotting was performed using commercially available antibodies from Abcam (Cambridge, MA): NDP52 (1:500); BD Biosciences (San Jose, CA): P62 (1:1000); Cell Signaling Technology (Danvers, MA): Beclin-1 (1:750), Caspase 3 (1:500), Caspase 9 (1:1000), GAPDH (1:40,000), LC3 (1:1000), mTOR (1:1000), NBR1 (1:500), phospho-AKTSer473 (1:1000), phospho-mTORSer2448 (1:500), phospho-P70 S6 KinaseThr389 (1:1000), phospho-ULKSer757 (1:1000), RAB7 (1:1000), and TAX1BP1 (1:1000); Novus Biologicals (Littleton, CO): PINK1 (1:250); Proteintech Group, Inc. (Rosemont, IL): Optineurin (1:500); Thermofisher Scientific (Waltham, MA): TFEB (1:500); and Santa Cruz Biotechnology (Dallas, TX): HSP60 (1:5000).
Cheng G., Hardy M., Zielonka J., Weh K., Zielonka M., Boyle K.A., Abu Eid M., McAllister D., Bennett B., Kresty L.A., Dwinell M.B, & Kalyanaraman B. (2020). Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. Cancer treatment and research communications, 25, 100210.
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2

Immunoblotting of Autophagic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 15 μg of protein was loaded in precast 4–20% Criterion TGX gels (Bio-Rad Laboratories, Hercules, CA), transferred to a PVDF membrane with the Trans-BlotⓇ Turbo™ system (Bio-Rad Laboratories), blocked, incubated with primary antibodies, and incubated with the secondary antibody. Images were captured via the ChemiDoc Molecular Imager and bands were quantified with ImageLab analysis software (both from Bio-Rad). Expression levels were determined by chemiluminescent immunodetection and normalized to appropriate loading controls. Immunoblotting was performed using commercially available antibodies from Abcam (Cambridge, MA): NDP52 (1:500); BD Biosciences (San Jose, CA): P62 (1:1000); Cell Signaling Technology (Danvers, MA): Beclin-1 (1:750), Caspase 3 (1:500), Caspase 9 (1:1000), GAPDH (1:40,000), LC3 (1:1000), mTOR (1:1000), NBR1 (1:500), phospho-AKTSer473 (1:1000), phospho-mTORSer2448 (1:500), phospho-P70 S6 KinaseThr389 (1:1000), phospho-ULKSer757 (1:1000), RAB7 (1:1000), and TAX1BP1 (1:1000); Novus Biologicals (Littleton, CO): PINK1 (1:250); Proteintech Group, Inc. (Rosemont, IL): Optineurin (1:500); Thermofisher Scientific (Waltham, MA): TFEB (1:500); and Santa Cruz Biotechnology (Dallas, TX): HSP60 (1:5000).
Cheng G., Hardy M., Zielonka J., Weh K., Zielonka M., Boyle K.A., Abu Eid M., McAllister D., Bennett B., Kresty L.A., Dwinell M.B, & Kalyanaraman B. (2020). Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. Cancer treatment and research communications, 25, 100210.
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